摘要
目的:探讨MMP-3和VEGF蛋白在胰腺癌组织中的表达及其与胰腺癌临床病理特征、预后及胰腺癌组织中血管新生的关系.方法:用免疫组织化学检测了56例手术切除的胰腺癌组织和正常胰腺组织中MMP-3、VEGF的蛋白表达和微血管密度,并请相关专业人员进行微血管密度计数分析.结果:在56例胰腺癌标本中有42例MMP-3(75.00%)和38例VEGF(67.85%)蛋白的表达阳性,明显高于正常胰腺组织.MMP-3和VEGF的阳性表达呈正相关(χ2=30.42,P<0.01).MMP-3在临床分期较晚和有淋巴结转移的胰腺癌中呈高表达.56例胰腺癌组织中的微血管密度与MMP-3、VEGF蛋白的阳性表达有关(tVEGF=3.67,P<0.01;tMMP-3=3.05,P<0.01).结论:MMP-3和VEGF可能通过相互促进的表达,促进胰腺癌组织中新生血管的形成,可能在胰腺癌的发生发展中起到重要作用.MMP-3对预测肿瘤的早期转移有一定的临床意义.而VEGF的相对低表达与临床所见胰腺癌在增强CT的低密度低动脉供血可能有关联.
AIM：To investigate the expression of vascular endothelial growth factor（VEGF）and matrix metalloproteinases-3（MMP-3）in pancreatic carcinoma（PC）and to analyze their relationship with tumor angiogenesis,invasion and metastasis.METHODS：The protein expression of VEGF and MMP-3 and microvessel density（MVD） were detected by immunohistochemistry in 56 PC specimens and 56 normal pancreatic tissue specimens.RESULTS：The positive rates of both MMP-3 and VEGF were significantly higher in PC than in normal pancreatic tissue（75.00%vs 3.57%,67.85%vs 1.78%,both P〈0.01）.There was a positive correlation between the expression of VEGF and that of MMP-3.Higher expression of MMP-3 was detected in late-stage PC and PC with lymph node metastasis.The expression of VEGF was correlated with clinical stage（P 〈0.05）.MVD was correlated with expression of MMP-3 and VEGF in PC（both P〈0.05）.CONCLUSION：MMP-3 and VEGF may promote tumor angiogenesis and play an important role in the development and progression of PC.Detection of MMP-3 expression could be used to predict early metastasis of PC.Lower expression of VEGF may be related to lower arterial blood supply in PC.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第15期1574-1578,共5页
World Chinese Journal of Digestology
基金
黑龙江省自然科学基金资助项目
No.ZJY0704-01~~
关键词
基质金属蛋白酶3
血管内皮生长因子
胰腺癌
Matrix metalloproteinases-3
Vascular endothelial growth factor
Pancreatic carcinoma
