摘要
以八仙花的茎段、茎尖、叶片为外植体,在MS培养基中加入不同质量分数的6-苄基腺嘌呤（6-BA）和吲哚丁酸（IBA）进行增殖培养.结果表明：适宜八仙花外植体表面灭菌的方法是利用0.1%氯化汞（HgC l2）灭菌7 m in;不同类型的八仙花外植体在初代培养时的增殖效果不同,茎尖在培养过程中首先伸长生长,然后陆续长出侧芽,表明八仙花的茎尖是比较适合用来进行增殖培养的外植体;继代培养以MS＋6-BA 1.0 mg.L-1＋IBA0.12 mg.L-1为较适宜的增殖培养基;侧芽在1/2 MS＋IBA 0.2 mg.L-1培养基中生根率和单苗根数量均较高,说明1/2 MS＋IBA 0.2 mg.L-1为较好的不定根诱导培养基.
Hydrangea macrophylla propagation were studied stem or leaf in culture medium with different 6-BA or IBA.The result showed：the good method for surface sterilization of explant was soaking them in 0.1% HgCl2 for 7min.Propagation was different because of explant types.Bud stem was good explant for propagation because it grows first and regenerates lateral bud.The propagation medium of Hydrangea macrophylla was MS＋6-BA 1.0 mg·L-1 ＋ IBA 0.12 mg·L-1.There were higher rooting rate and more adventitious roots in 1/2 MS＋IBA 0.2 mg·L-1.It was good medium to induce adventitious root of Hydrangea macrophylla.
出处
《北华大学学报（自然科学版）》
CAS
2011年第3期350-352,共3页
Journal of Beihua University（Natural Science）
关键词
八仙花
组织培养
繁殖
Hydrangea macrophylla
tissue culture
propagation
