摘要
目的探讨胰岛素是否通过细胞外信号调节激酶(ERK)通路调控糖尿病模型大鼠动脉壁肌球蛋白轻链激酶(MLCK)的表达。方法 SD大鼠45只,随机分为正常对照组、高脂对照组、糖尿病组、胰岛素治疗组,通过腹腔注射链尿佐菌素(STZ)复制糖尿病大鼠模型,心脏取血检测血清学指标,采用免疫组化法和Western blot法分析大鼠动脉壁MLCK的表达以及肌球蛋白轻链(MLC)、ERK表达及其磷酸化水平。结果正常对照组动脉壁中MLCK的表达处于较低水平,高脂对照组表达略有升高,而糖尿病组MLCK表达较正常对照组和高脂对照组明显升高,差异有统计学意义(P<0.05),胰岛素治疗可以显著降低糖尿病造成的MLCK表达的升高(P<0.05);并且MLC、ERK的磷酸化水平与MLCK表达呈一致性。结论糖尿病进程中可能通过ERK通路上调动脉壁MLCK的表达而导致血管损伤,而胰岛素治疗可以下调动脉壁MLCK的高表达。
Objective To explore the possibility that the expression of myosin light chain kinase （MLCK） in the artery wall of diabetic rats is regulated by the insulin through the pathway of extraeellular signal-regulated kinase （ERK）. Methods Forty-five SD rats were randomly divided into control group, high-lipid diet group, diabetic group and insulin group. The diabetic rat model was induced by intraperitoneal injection of streptozotoein （STZ）. The serum glucose and lipid levels were measured each group. The expression of MLCK and the level of phosphorylated MLC and ERK were detected by Western blot and immunohistochemical methods. Results The expression of MLCK in the artery of diabetic group increased markedly compared with that of control and high-lipid diet group （P 〈 0. 05）. After treated with insulin for 12 weeks, the expression of MLCK decreased compared with that of diabetic group（P 〈 0. 05）. In addition, the level of phosphorylated MLC and ERK showed the same pattern as MLCK. Conclusion During the onset of diabetes, the expression of MLCK in the artery might be up-regulated through ERK signal pathway which lead to blood vessel damage. However, insulin therapy could down-regulate ab- normal MLCK expression in the artery.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第6期519-523,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:30971226)
安徽省自然科学基金(编号:090413091)
