摘要
目的 建立血糖钳方法 ,检验所建高脂、高果糖饮食诱发的胰岛素抵抗模型以及谷氨酸钠 (monosodiumglutam ate,MSG)所致的肥胖性大鼠胰岛素抵抗模型。方法 腹腔注射小剂量链脲霉素 (streptozotocin,STZ) ,30 mg/ kg轻度损伤胰岛β细胞 ,造成大鼠糖耐量异常后加喂高果糖高脂肪饲料 ,并饮用 10 %果糖水 ,造成胰岛素抵抗。吡格列酮2 0 mg/ kg·d- 1治疗后 ,以血糖钳方法测定葡萄糖输注速率 (glucose infusion rate,GIR) ;另一模型为具胰岛素抵抗特征的 MSG肥胖性大鼠。新生 Wistar大鼠出生第二天起皮下注射 L-谷氨酸钠 4g/ kg·d- 1 ,连续 7天 ,造成肥胖。10月龄时 ,用中药复方千金黄连丸加味提取物 ,相当于生药 10 g/ kg· d- 1 治疗 ,并以血糖钳方法测定对葡萄糖输注速率的影响 ,且分析了反映胰岛素敏感性的 GIR值同血脂、脂肪重、体重、血胰岛素水平及胰岛素敏感指数等指标的相关性。结果 两模型组同相应正常对照组相比 ,胰岛素敏感性均明显降低 ,给药后均有显著提高。且 GIR反映的敏感性同胰岛素敏感指数呈较好的正相关 ,而与空腹血胰岛素水平、血甘油三酯、血总胆固醇以及腹脂重、腹脂 /体重值呈负相关。结论 高脂高果糖饮食诱发的大鼠模型及 MSG肥胖性大鼠均具有明显的胰岛素抵抗 ,分别以吡格列酮?
Objective To set up the method of euglycemic clamp and evaluate insulin resistance having developed in high fat high fructose induced rats and monosodium glutamate (MSG) induced obese rats.Methods Female Wistar rats were injected a low dose of streptozotocin (30mg/kg) intraperitoneally followed by a high fat high fructose diet and drank 10% fructose water. After feding for about 6 weeks, pioglitazone, an insulin sensitizer, was administered at the dose of 20mg/kg/day and insulin sensitivity was measured with euglycemic clamp technique. The second model was MSG obese rats. Neonatal rats were treated with monosodium glutamate, when 10 months old, the extract of Traditional Chinese Medicine recipe qianjinhuanglianwanjiawei (equal to raw medicinal plant 10g/kg/day) were given for one month, then insulin sensitivity was estimated by euglycemic clamp method in all groups. Moreover, the correlation between glucose infusion rate (GIR) and serum lipid level, adipose tissue weight, body weight, the ratio of adipose weight and body weight, serum insulin level, and insulin sensitivity index (ISI) were respectively analyzed.Results GIR was significantly decreased in the two model rats and improved to the normal level after agents treatment. GIR was well positively correlated with ISI, suggesting they both well reflect the insulin sensitivity.Conclusion It was proved by euglycemic clamp technique that the two models both have the characteristics of significant insulin resistance. Pioglitazone and the extract of the TCM recipe can enhance insulin sensitivity on the two models respectively.
出处
《中国糖尿病杂志》
CAS
CSCD
2001年第5期286-289,共4页
Chinese Journal of Diabetes
基金
"九五"国家攀登计划预选项目的资助 No970 2 1 1 0 1 8
关键词
血糖钳
胰岛素抵抗
高脂饮食
糖尿病
Glucose clamp technique Insulin resistance High fat diet
