摘要
目的:探讨环氧合酶2(COX-2)抑制剂塞来昔布对胃癌SGC7901细胞株的放射增敏作用及其机制。方法:MTT法检测塞来昔布对胃癌SGC7901细胞株的抑制作用,计算出塞来昔布的半数抑制浓度(IC50);克隆形成实验用于检测20%IC50这个浓度的塞来昔布对胃癌SGC细胞是否具有放射敏感性;流式细胞术(FCM)分析细胞周期的分布情况。结果:MTT实验显示塞来昔布对SGC7901细胞株的抑制率随浓度的升高而升高,48 h的IC50是34.38μmol/L;克隆形成实验显示,照射组+药物组与单纯照射组相比,反映放射敏感性指标的存活分数(SF2)、平均致死剂量(D0)及准阈剂量(Dq)均下降,放射增敏比(SER)升高。FCM检测细胞周期G2和M期细胞比例增加,S期细胞比例减少。结论:塞来昔布能增加胃癌SGC7901细胞的放射敏感性,其机制可能与其抑制肿瘤细胞亚致死性损伤修复能力和促进肿瘤细胞周期再分布有关。
OBJECTIVE： To investigate the radiosensitivity enhancement effect of cyclooxygenase-2（COX-2） inhibitor celecoxib on gastric cancer cells in vitro and to initially disclosure its mechanism.METHODS：MTT assay was used to measure the proliferation inhibition of celecoxib on SGC7901 cells and determine the concentration IC50.The clonogenic assay was performed to determine the radiosensitizing effect of celecoxib on gastric cancer cells with the concentration of 20% IC50.The cell cycle distribution was analyzed by flow cytometry（FCM）.RESULTS： Inhibition of cell proliferation seemed more dependent on the dose and the time.The IC50 of 48 h was 34.38 μmol/L.The group of radiation combined with celecoxib displayed the value of SF2,D0,Dq,SER was significantly lower than those of radiation alone group.Cell cycle analysis showed that the cell population in G2/M phase increased and in S phase decreased.CONCLUSIONS： COX-2 inhibitor celecoxib can obviously enhance the radiosensitivity of SGC7901 cells,which is probably associated with the inhibition of sublethal damage of tumor cells and facilitation of redistribution of tumor cell cycle.
出处
《中华肿瘤防治杂志》
CAS
2011年第4期260-263,共4页
Chinese Journal of Cancer Prevention and Treatment
关键词
塞来昔布
胃肿瘤
放射增敏
放射治疗比
celexcoxib
stomach neoplasms
radiosensitivity
therapeutic ratio
