摘要
目的探讨塞来昔布抑制非小细胞肺癌肿瘤增殖及血管生成的效果。方法 48只裸鼠建立非小细胞肺癌模型后,分为两组,均使用含有塞来昔布的饲料喂养,观察组则联合使用浓度为100μmol/L的塞来昔布治疗,持续120d后,比较两组细胞增殖情况及血管内皮生长因子水平和微血管密度。结果观察组72h抑瘤率高于对照组(P<0.05),细胞凋亡染色率(TUNEL法)高于对照组(P<0.05),血管内皮生长因子整体水平低于对照组(P<0.05),微血管密度低于对照组。结论对非小细胞肺癌大鼠,使用100μmol/L塞来昔布治疗能较好的抑制肺癌肿瘤细胞的增殖以及新生血管的形成,且随着时间的延长,抑瘤率提高。
Objective To explore the effect of celecoxib in the inhibition of proliferation of non-small cell lung cancer and angiogenesis. Methods 48 nude mice were used to establish non-small cell lung cancer model and divided into two groups. All the mice were using the feed containing celecoxib. On this basis, mice in observation group were also treated with celecoxib with concentration of 100 μmol/L. After 120 days, cell proliferation, vascular endothelial growth factor and microvessel density of the two groups were compared. Results 72 hours of tumor suppression rate of observation group was higher than that of control group （P 〈 0.05）. Apoptosis rate （TUNEL method） of observation group was higher than that of control group （P 〈 0.05）. Total level of vascular endothelial growth factor of observation group was lower than that of control group （P 〈 0.05）. Vascular density of observation group was lower than that of control group （P 〈 0.05）. Conclusion For non-small cell lung cancer rats, celecoxib with concentration of 100 μmol/L can inhibit the proliferation of lung cancer cells and the formation of new blood vessels. And with the extension of time, the tumor suppression rate increases.
出处
《中国医药科学》
2016年第15期31-33,43,共4页
China Medicine And Pharmacy
关键词
塞来昔布
非小细胞肺癌
肿瘤增殖
血管生成
实验研究
Celecoxib
Non-small cell lung cancer
Tumor proliferation
Angiogenesis
Experimental study
