摘要
该文利用免疫组化检测了４０ 例霍奇金病（ ＨＤ） 中ＲＳ／ Ｈ 细胞ｂｃｌ－ ２ 、ｆａｓ 及ｃｙｃｌｉｎ Ｄ１ 的表达，并对其相关关系进行了研究。结果显示：ｂｃｌ － ２ 和ｆａｓ 蛋白阳性信号在ＲＳ／ Ｈ 细胞中主要呈浆膜型。在ＨＤ 四种亚型ＬＰ，ＮＳ，ＭＣ 和ＬＤ 中ｂｃｌ－ ２ 阳性细胞密度逐渐增高（ Ｐ＜ ０ ．０５） ，而ｆａｓ 阳性细胞密度依次降低（ Ｐ＜ ０ ．０５） ；并呈明显负相关（ｒ ＝ － ０ ．５６９０） 。在４０ 例ＨＤ 中ｃｙｃｌｉｎ Ｄ１ 阳性表达率为７０ ％ （２８／４０） ，阳性信号主要位于核内；ｃｙｃｌｉｎ Ｄ１ 阳性病例中的ｂｃｌ－ ２ 阳性细胞密度显著高于ｆａｓ 阳性细胞密度（ Ｐ＜ ０ ．０１） ，而ｃｙｃｌｉｎ Ｄ１ 阴性病例中两者无显著差异（ Ｐ＜０ ．０５） ；在ｃｙｃｌｉｎ Ｄ１ 阳性和阴性病例中，ｂｃｌ － ２ 和ｆａｓ 阳性细胞密度均呈明显负相关（ 分别ｒ ＝ － ０ ．６１３１ ，ｒ ＝－ ０ ．７３１０） 。提示抗凋亡基因ｂｃｌ－ ２ 和促凋亡基因ｆａｓ 的表达与ＨＤ 不同组织学亚型及预后有关；ｃｙｃｌｉｎ Ｄ１ 蛋白可能具有促进ｂｃｌ － ２ 的表达和／ 或抑制ｆａｓ 表达作用，从而导致ＲＳ／ Ｈ
The expressions of bcl-2,fas and cyclin D1 protein were detected by immunohistochemistry in RS/H cells of 40 cases of Hodgkin's disease(HD),and their correlationshipsn were analyzed.The results showed that the positive expressions of bcl-2 and fas proteins were mainly cytoplasm-membrane type in RS/H cells.The density of positive cells for bcl-2 protein increased gradually(P<0.05),while the density of fas protein decreased gradually(P<0.01); both densities were negatively correlated in linear fashion(r=-0.5690) in the subtypes of HD(LP,NS,MC and LD).The positive rate of cyclin D1 was protein expression in 40 cases of HD was 70%(28/40).The positive expression of cyclin D1 was located in the nuclei of the RS/H cells.In the positive cases of cyclin D1 protein, the density of bcl-2 positive cells was significantly higher than that of the fas positive cells (P<0.01);a correlation between bcl-2 expression and fas expression was found in the cases of negative cyclin D1 protein (P<0.05).There was a negative correlation between the densities of bcl-2 and fas positive cells both in the cases of positive cyclin D1 protein(r= 0.6131)and negative cyclin D1 protein(r=-0.7310).The results suggest that the expression of bcl-2 and fas proteins may be associated with the subtype and prognosis of HD;and cyclin D1 overexpression may result in the imbalance of the proliferation and apoptosis of RS/H cells by promoting the expression of bcl-2 protein and/or reducing the expression of fas.
出处
《中国现代医学杂志》
CAS
CSCD
1999年第9期1-2,共2页
China Journal of Modern Medicine
基金
美国CMB 资助项目
关键词
霍奇金病
癌基因
免疫组化
Hodgkin's Disease
Oncogene
Immunohistochemistry
