摘要
研究利用胰酶粉提取胰激肽释放酶的静态提取工艺。分别考察了浸提pH值、平衡液pH值、吸附过程中的NaCl添加量、洗脱过程中pH值、NaCl添加量以及醋酸钠正交试验对利用胰酶粉静态提取胰激肽释放酶的影响。利用胰酶粉静态提取胰激肽释放酶的最佳条件为:浸提pH值为6.0,平衡液pH值为6.5,吸附过程中添加0.02mol/LNaCl,洗脱优化条件控制在pH值为6.5,NaCl0.35mol/L以及不添加醋酸钠,提取率达到76%以上,该静态条件的考察为胰激肽释放酶的进一步分离纯化提供了理论依据。
The aim of this study was to optimize static extraction of pancreatic kallikrein from trypsogen power. Orthogonal test was used for optimization, and the factors included extraction pH value, equilibrium pH value, amount of NaCl during adsorption process, elution pH value, amount of NaCl during elution process and CH3COONa. The optimal conditions for static extraction of pancreatic kallikrein from trypsogen power are as follows： extraction pH value, 6.0; equilibrium pH value, 6.5; 0.02 mol/L NaCl during adsorption process; elution pH value, 6.5; 0.35 mol/L during elution process; and no addition of CH3COONa. The extraction rate was more than 76%. This study provides a theoretical basis for further study on separation and purification of pancreatic kallikrein.
出处
《畜牧与饲料科学》
2011年第4期5-7,共3页
Animal Husbandry and Feed Science
基金
浙江省新苗人才计划(2010R428031)
关键词
胰酶粉
胰激肽释放酶
胰蛋白酶
分离纯化
trypsogen power
pancreatic kallikrein
trypsinase
separation and purification
