摘要
为分析食用菌中海藻糖的含量,以蛹虫草子实体为材料,比较了提取溶剂、提取方式及提取时间等条件对海藻糖提取效果的影响,确定海藻糖分析的前处理方法为:1g子实体粉中加入100mL90%乙醇热回流提取1h。采用高效液相色谱法测定海藻糖,优化后的色谱条件为:SUGAR SP0810柱(300mm×8mm),超纯水洗脱,流速0.5mL/min,柱温70℃,示差折光检测器检测,进样量10μL。方法学考察结果表明,该方法准确度高,稳定性、精密度、重现性好,对海藻糖标准品的检测特异性良好,适用于蛹虫草子实体中海藻糖含量的分析。
In order to determine the content of trehalose in mushroom,a procedure of trehalose extraction from Cordyceps militaris was developed.The results proved that the optimal method for extraction of trehalose was as follows： 1g of powder of Cordyceps militaris fruiting bodies was extracted by reflux in 100mL 90% ethanol for 1h.Then a high performance liquid chromatography （HPLC） was developed for the determination of trehalose.By use of SUGAR SP0810 column （300mm？8mm） as the chromatographic column and purified water as mobile phase,trehalose was separated completely at the refractive index detector with the flow rate of 0.5mL/min at the temperature of 70℃.The injection volume was 10μL.The methodological study showed that the method was sensitive,accurate,reproducible and successfully applied to analyze the trehalose in Cordyceps militaris.
出处
《菌物学报》
CAS
CSCD
北大核心
2011年第3期472-476,共5页
Mycosystema
基金
上海市农委重点攻关项目[沪农科攻字(2009)第2-2号]
