摘要
目的研究HIV感染者肠黏膜组织中CD4^＋CD25^＋调节性T淋巴细胞（Treg）及叉头状转录因子3（FoxP3）的表达。方法应用免疫组织化学法，检测21例HIV感染者及17例非HIV感染者肠黏膜组织中CD4^＋CD25^＋Treg的特异性标志物FoxP3的表达以及CD4^＋T淋巴细胞的原位表达。数据处理应用￡检验。结果HIV感染者肠黏膜组织中CD4^＋T淋巴细胞阳性标记指数为11．56％土4．44％，显著低于非HIV感染者的43．49％±8．90％（t=11．86，P〈0．01）。H1V感染者肠黏膜组织中FoxP3阳性标记指数为0．46％±0．20％，显著低于非HIV感染者的1．18％±0．44％（f=15．98，P〈0．01）。结论HIV感染者肠黏膜组织中CD4^＋T淋巴细胞出现消减，CD4^＋CD25^＋Treg也出现消减，FoxP3表达下降。
Objective To investigate the changes of CD4＋ CD25＋ regulatory T lymphocyte （Treg） and expressions of folkhead helix transcription factor 3 （FoxP3） in intestinal mucosa in human immunodeficiency virus （HIV） infected patients. Methods Twenty one HIV infected patients and 17 control subjects without HIV infection were included in this study. The expression of FoxP3, which was considered as a specific marker of CD4＋ CD25＋ Treg, was detected in intestinal mucosa specimens from HIV infected patients by immunohistochemistry. Meanwhile, the in situ expression of CD4＋ T lymphocyte was also determined by immunohistochemistry. The data were analyzed by t test. Results The positive labeling index of CD4 ＋ T lymphocyte in intestinal mucosa was significantly lower in HIV infected patients compared to the controls （11.56%±4.44% vs 43.49^±8.90% ,t =11.86, P〈0.01）. The positive labeling index of FoxP3 in intestinal mucosa was also significantly lower in HIV infected patients compared to the controls （0.46%± 0. 20% vs 1.18%± 0.44%,t= -5.98, P〈0.01）. Conclusion The depletion of CD4＋ CD25＋ Treg is accompanied with the depletion of CD4＋ T lymphocyte and the reduction of FoxP3 expression in intestinal mucosa of HIV infected patients.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2011年第5期300-303,共4页
Chinese Journal of Infectious Diseases
