摘要
目的研究AIDS患者肺组织巨细胞病毒(CMV)感染与CD4^+CD25^+调节性T淋巴细胞表达的关系。方法应用免疫组织化学方法,检测15例AIDS尸检患者肺门淋巴结中CD4^+CD25^+调节性T淋巴细胞的特异性标记物叉头/翅膀状螺旋转录因子(FOXP3)的表达,同时应用免疫组织化学和核酸原位杂交方法检测肺组织中CMV抗原及核酸的表达。结果本组病例中有8例表现为肺部CMV感染。CMV肺炎主要表现为间质性肺炎及巨细胞包涵体形成;肺部CMV感染常合并其他机会性感染;应用CMV单克隆抗体免疫组织化学及原位杂交技术可明确CMV感染诊断。CMV感染者FOXP3阳性标记指数显著高于无感染者;CMV感染者CD4^+、CD8^+淋巴细胞计数略低于无感染者,但差异无统计学意义。结论本组病例AIDS患者肺部CMV感染率较高,可能与调节性T淋巴细胞的高表达有关。
Objective To investigate the correlation of cytomegalovirus （CMV） infection and the expression of CD4^＋CD25^＋ regulatory T lymphocytes in lung specimens of AIDS patients. Methods The detection of forkhead/winged helix transcription factor （FOXP3）, which was considered as a specific marker of CD4^＋CD25^＋regulatory T lymphocytes of lung lymph nodes, was performed in 15 patients with AIDS by immunohistochemistry, meanwhile the antigen and DNA of CMV were detected by immunohistuchemistry with monoclonal antibody and in situ hybridization, respectively. Results Eight of 15 patients showed pulmonary CMV infection, with the manifestations of interstitial pneumonia and giant-cell inclusion formation, complicated by other opportunistic infections. The diagnosis of CMV infection was confirmed via immunohistochemistry with specific monoclonal antibody and in situ hybridization. The positive label index of FOXP3 in AIDS patients with CMV infection was significantly higher than that in AIDS patients without CMV infection （0.99% ± 0.06% vs 0.42% ± 0.16%, P〈 0.01）, while CD4^＋ and CD8^＋ T lymphocyte counts in AIDS patients with CMV infection were lower than those in AIDS patients without CMV infection, but the differences were not significant between the two groups. Conclusion High pulmonary CMV infection in AIDS patients in this study may be related to higher expression of CD4^＋CD25^＋ regulatory T lymphocytes.
出处
《传染病信息》
2008年第5期291-293,307,共4页
Infectious Disease Information
基金
国家"973"计划项目(2006CB504201)
北京市科委研发攻关类项目(D0906003040291)
