摘要
目的研究Wnt信号途径的抑制基因(sfrp1、sfrp2、sfrp5)在侧向发育型肿瘤(LST)及隆起型腺瘤(PA)中的表达情况及甲基化状态。方法运用半定量PCR法和免疫组织化学方法研究52例LSTs和51例PAs组织标本sfrp1、sfrp2、sfrp5表达情况;运用甲基化特异性PCR法(MSP)对3个抑制基因甲基化状态进行研究。结果 LSTs组sfrp1mRNA及蛋白表达水平均比PAs组显著降低(P<0.05),但sfrp2及sfrp5mRNA表达在LSTs组和PAs组中无显著差异(P>0.05)。LSTs组中的sfrp1基因存在高甲基化(P<0.05),sfrp2及sfrp5基因不存在高甲基化(P>0.05)。结论 sfrp1表达缺失或下调在LSTs比PAs更常见,该基因高甲基化发生频率在LSTs也相应比PAs高,提示LSTs的发病机制可能与PAs存在不同。
Objective To investigate the expression and methylation of Wntantagonist genes on laterally spreading tumors （LSTs） and protruding adenomas （PAs）.Methods We investigated the expression of key molecules （sfrp1,2,5 genes） in Wnt signaling pathway by semi-quantitative RT-PCR and immunostaining techniques.The methylation of Wnt antagonist genes in tissues （consisted of 52 LSTs and 51 PAs） was analysed by methylation specific PCR （MSP）.Results The expression of sfrp1 mRNA in LST group was significantly lower than that in PA group （P 0.05）.Meanwhile,protein expression of sfrp1 was significantly decreased in LST compared with those in PA （P 0.05）.As for sfrp2 and sfrp 5 mRNA expression,there was no significant difference between LST group and PA group （P 0.05）.The methylation of sfrp1 in LST group was significantly higher than that in PA group （P 0.05）.However,there was no significant difference in the methylation of sfrp 2 and sfrp 5 between LST group and PA group （P 0.05）.Conclusion Absence or downregulation of sfrp1 expression might play a role in the progression of LST.Hypermethylation of sfrp1 was an important mechanism for downregulation of sfrp1 expression.
出处
《现代消化及介入诊疗》
2011年第2期105-108,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
