摘要
应用MassARRAY DNA甲基化定量分析方法检测58例宫颈癌组织、57例CIN2/3组织、36例CIN1组织及28例正常对照组织中SFRP1基因启动子区各CpG位点甲基化,探讨SFRP1基因启动子区甲基化与宫颈癌演进及与HPV16感染的相关性。结果显示:2110个CpG单位中甲基化率低于30%的CpG单位1612个,占总数的76.4%;甲基化率大于80%的CpG单位为4.4%,多分布于CIN2/3和宫颈癌组织之中。其中SFRP1基因启动子区CpG12.13和CpG18位点的甲基化率宫颈癌组高于对照、CIN1和CIN2/3组,差异具有统计学意义(P<0.05)。CpG12.13和CpG18位点甲基化率与HPV16感染无相关性(P>0.05)。提示SFRP1基因在CpG12.13和CpG18位点上高甲基化可能与宫颈癌的演进相关。
The paper is to explore the correlations of secreted frizzled related protein 1（SFRP1） gene methylation and cervical carcinogenesis.The status of SFRP1 gene was detected by using the Sequenom MassARRAY method in cervical squamous cell carcinoma（n=58）,cervical intraepithelial neoplasia（CIN;CIN1 n=36;CIN2/3 n=57）,and normal cervical tissue（n=28）.The analyzed regions of the SFRP1 gene contained 15 CpG units（total of 2,110 analyzed units in 157 samples）.Nearly 76.4% of CpG units were methylated less than 30% in the 2110 CpG units,and 4.4%CpG units more than 80%,which mostly in CIN2/3 and cervical squamous cell carcinoma tissues.The levels of methylation of CpG12.13 and CpG18 sites in tumors was significantly higher than in normal,CIN1,and CIN2/3 samples（P〈0.05）.No significant differences were found in the methylation levels of the CpG12.13 and CpG18 sites associated with HPV16 infection in cervical squamous cell carcinoma and CIN2/3（P〉0.05）.Our data suggest that CpG12.13 and CpG18 sites hypermethylation of SFRP1 gene are associated with cervical carcinogenesis.
出处
《石河子大学学报（自然科学版）》
CAS
2010年第3期325-329,共5页
Journal of Shihezi University(Natural Science)
基金
国家科技支撑计划项目(2009BA182B03)
