摘要
目的了解北京地区与杭州地区宋内志贺菌的耐药状况和基因型的特征。方法用常规方法培养、分离细菌,经生化反应和血清凝集试验鉴定到种。用Kirby-Bauer纸片法进行药物敏感性试验。超广谱β-内酰胺酶(extended-spectrumβ-lactamases,ESBLs)检测采用双纸片确认试验。采用PCR方法进行基因扩增。结果从北京地区分离获得203株宋内志贺菌,对氨苄西林、复方磺胺甲噁唑、头孢曲松的耐药率分别为56.70%、94.00%、19.10%。从杭州地区分离获得97株宋内志贺菌,耐药率较高的抗生素也是氨苄西林、复方磺胺甲噁唑、头孢曲松,但耐药率分别为99.00%、91.10%和71.30%。北京地区有43株产ESBLs,占21.18%,其耐药基因为CTX-M1群、CTX-M9群及TEM基因,分别占37.21%、25.58%和41.86%;杭州地区有73株产ESBLs,占75.26%,其耐药基因为CTX-M1群、CTX-M9群及TEM、OXA基因,分别占17.81%、58.90%和45.21%、9.59%。结论北京和杭州地区宋内志贺菌对氨苄西林、头孢菌素等抗生素耐药性有显著差异,并检测到产ESBLs,主要耐药基因包括CTX-M1群、CTX-M9群及TEM、OXA基因。
Objective To investigate the drug resistance of ShigeUa sonnei isolates and the characteristics of drug resistance genes in Hangzhou and Beijing. Methods Bacterial cultivation and isolation were carried out using conventional techniques. Bio- chemical reactions and serum agglutination test were performed for species identification. Antimicrobial drug susceptibility was de- termined using Kirby-Bauer antibiotic testing. Extended-spectrum β-lactamases （ESBLs） were detected using double disk diffusion test. Polymerase chain reaction was performed for gene amplification. Results A total of 203 ShigeUa sonnei strains were isolated from diarrheal patients in Beijing with high levels of resistance to ampicillin （56.70%）, trimethoprim-sulfamethoxazole （94.00%） and ceftriaxone （19.10%）. A total of 97 Shigella sonnei strains were isolated from diarrheal patients in Hangzhou with high levels of resis- tance to ampicillin （99.00%）, trimethoprim-sulfamethoxazole （91.10%） and ceftriaxone （71.30%）. Forty-three （21.18%） isolates in Beijing produced ESBLs, with CTX-M1 group accounting for 37.21%, CTX-M9 group 25.58% and TEM gene 41.86%. Seventy- three （75.26%） isolates in Hangzhou produced ESBLs, with CTX-M1 group accounting for 17.81%, CTX-M9 group 58.90%, TEM gene 45.21% and OXA gene 9.59%. Conclusions The resistance of Shigella sonnei to ampieillin and cephalosporin is significantly different in Beijing and Hangzhou. Some isolates are detected to produce ESBLs, and the main susceptibility genes are CTX-MI group, CTX-M9 group, TEM gene and OXA gene.
出处
《传染病信息》
2011年第2期82-84,93,共4页
Infectious Disease Information
基金
总后勤部多重耐药菌紧急启动课题(10YJ003)
