摘要
目的：建立HPLC法测定藏药二十五味珊瑚丸中乌头碱的限量。方法：采用Phenomenex Gemini DOS（4．6mm×250mm，5μm）色谱柱，以0．04mol·L^-1三乙胺（用磷酸调节至pH3．0±0．1）-甲醇（60：40）为流动相，流速1．0mL·min^-1，检测波长235nm，柱温40℃。结果：乌头碱在1～120μg范围内，存在良好的线性关系（r=0．9998）；检测限为10ng。结论：本方法快速简便，准确可靠，可用于二十五味珊瑚丸的质量控制。
Objective： To establish the HPLC method of determing aconitine in Ershiwuwei Shanhu Pills. Methods： The Phenomenex Gemini DOS （ 4. 6 mm×250mm,5μm）was used with a mobile phase of triethylamine（adjusted to pH 3.0±0. 1 by phosphoric acid ）-methanol（60： 40）, the wavelength of detection was set at 235 nm, the flow rate was 1.0 mL ·min-1 with column temperature at 40℃. Results： The calibration curves were linear in the rang of 1 - 120 μg （ r =0. 999 8） for aconitine. The limits for detection were 10 ng. Conclusion： The method is simple, reliable and reproducible and can be used effectively for the quality control of Ershiwuwei Shanhu Pills
出处
《中国药品标准》
CAS
2011年第2期99-101,共3页
Drug Standards of China
