摘要
目的：建立了反相离子对色谱法测定益母草中盐酸益母草碱含量的方法。方法：Agilent SB—C18柱（4．6mm×250mm，5μm）；流动相：乙腈-0．4％辛烷磺酸钠的0．1％磷酸溶液（24：76）；检测波长：277nm；流速：1．0mL·min-1。结果：盐酸益母草碱进样量在0．02866～1．433μg范围内呈良好的线性关系，平均回收率为98．0％，RSD为0．6％。结论：此方法简便、灵敏度高，重现性好，可用于益母草的质量控制。
Objective： A method was developed for determination of leonurine hydrochloride in Herba Leonuri by reversed-phase ion-pair chromatography. Methods： The separation was performed on an Agilent SB-C18 column （4.6 mm ×250 mm,5μm） with acetonitrile-0. 4% sodium octanesulfonate of 0. 1% phosphoric acid solution （24： 76） as mobile phase at a flow rate of 1.0 mL·min-1 The detection wavelength was 277 nm. Results： There was a good linear relationship with the range of 0. 028 66-1. 433μg. The average recovery was 98.0% （ RSD =0. 6% ） . Conclusion： The method is simple, sensitive and reliable, it can be used as quantitative analysis method for Herba Leonuri.
出处
《中国药品标准》
CAS
2011年第2期111-113,共3页
Drug Standards of China
基金
国家十一五"重大新药创立"专项(编号:2009ZX09308-001)
关键词
反相离子对色谱
益母草
盐酸益母草碱
Reversed-phase ion-pair chromatography
Leonurine hydrochloride
Herba Leonuri
