摘要
目的:通过稳定、高效、低毒的基因转染方法将nm23-H1基因导入该基因缺失的人肺大细胞癌株L9981,从基因及蛋白水平鉴定nm23-H1基因转染肺癌细胞的建立。方法:以JM109为宿主菌,转化质粒载体PCMV-neo-Bam-nm23-H1,制备nm23-H1基因的真核表达载体;利用阳离子脂质体Lipofectamine介导,将nm23-H1基因导入L9981细胞株。采用RT-PCR技术检测转染后nm23-H1基因的表达,并用Western blot技术检测转染后nm23-H1蛋白的表达。结果:转染后的L9981细胞株中可检测到nm23-H1 mRNA及nm23-H1蛋白的阳性表达。结论:建立表达nm23-H1基因的肺癌细胞株L9981,为研究nm23-H1对肺癌恶性转移表型的逆转作用作基础准备。
OBJECTIVE：nm23-H1 gene was transfected into nm23-H1 deletion human large lung cancer cell L9981 by stable,continuous and high effective gene transfection method.METHODS：nm23-H1 gene expression carrier was established,nm23-H1 was transfected in L9981 cell line and the expression of nm23-H1 was confirmed by gene transduction and protein translation.RESULTS：Transfected cell line showed nm23-H1 gene and protein expressions. CONCLUSION：The expression of nm23-H1 by L9981 cell line provides support for utilizing nm23-H1 as a potential therapy in lung cancer.
出处
《癌变·畸变·突变》
CAS
CSCD
2011年第2期145-147,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(30070333)
