摘要
背景与目的已有的研究表明硒具有防癌作用和抑制乳腺癌、前列腺癌细胞株增殖的作用,但有关甲基硒酸(MSA)是否具有抗肺癌的作用目前尚不清楚。本研究的目的是探讨MSA对人高转移大细胞肺癌细胞株L9981是否具有生长抑制及诱导凋亡的作用,并对其分子机制作初步探讨。方法应用体外细胞培养技术,以MSA处理L9981细胞株,应用台盼蓝计数法、克隆形成实验和流式细胞术检测MSA处理L9981细胞株前后细胞株体外增殖、克隆形成和凋亡水平的变化。应用流式细胞术检测MSA处理L9981细胞株前后细胞周期、细胞周期相关蛋白和凋亡相关蛋白表达水平的变化。结果①MSA在大于0.5μmol/L的浓度时可显著抑制L9981细胞株的增殖(P<0.05),细胞被阻滞于G0/G1期。②MSA浓度在2.5μmol/L时可诱导L9981细胞株进入凋亡(P<0.05)。③MSA在5μmol/L时可明显抑制L9981细胞株的克隆形成能力(P<0.05)。④MSA能显著上调P53、P21、Fas、FasL和Bax蛋白表达水平。结论①MSA可显著抑制人高转移大细胞肺癌细胞株L9981的体外增殖和克隆形成能力,并诱导细胞凋亡;②MSA的抗肺癌作用可能与其调控肺癌细胞株L9981细胞周期和细胞凋亡相关基因表达有关。
Background and objective It has been proved that selenium has remarkable effects in the prevention of cancer and proliferation inhibition for breast cancer and prostate cancer. Up to now, little is known, however, if methylseleninic acid （MSA） has the anticancer effect on lung cancer or not. The objective of this study is to detect the effect of MSA on proliferation inhibition and apoptotic induction for human high-metastatic large cell lung cancer cell line L9981, and to explore the molecular mechanisms. Methods The changes of proliferation, clone formation, apoptotic level and cell cycles were detected in L9981 by trypan blue staining, clone formation suppression test, and flow cytometry before and after treating with different concentration of MSA. The expression level of proliferative-related and apoptotic-related genes was also determined in L9981 by flow cytometry. Results ①The proliferation ability of L9981 was remarkably inhibited at the concentration of 0.5 μmol/L of MSA （P〈0.05）, and the cells were arrested at G0/G1 phase after treating with the same concentration. ② Apoptosis of L9981 was remarkably induced by MSA at the concentration of 2.5 μtmol/L （P〈0.05）. ③The clone formation ability of L9981 was significantly suppressed by MSA at the concentration of 5.0 μtmol/L （P〈0.05）. OThe expression levels of P53; P21, Fas, FasL and Bax were remarkably up-regulated after treatment with MSA. Conclusion ①MSA can significantly suppress the proliferation and clone formation ability of human high-metastatic large cell lung cancer cell line L9981, and also induce apoptosis of L9981. ②The anticancer effects of MSA might be related to regulate the expression of cell cycle-related genes and apoptotic-related genes in the human high-metastatic large cell lung cancer line L9981.
出处
《中国肺癌杂志》
CAS
2006年第2期103-108,共6页
Chinese Journal of Lung Cancer
关键词
甲基硒酸
人高转移大细胞肺癌细胞株L9981
生长抑制
凋亡
Methylseleninic acid Human high-metastatic large cell lung cancer line L9981 Growth inhibition Apoptosis
