摘要
目的 建立转染野生型nm2 3 H1 基因的人大细胞肺癌细胞株L9981 nm2 3 H1 。方法 应用基因克隆技术构建逆转录病毒载体pLXSN nm 2 3 H1 EGFP。脂质体法转染PA3 17细胞 ,得到逆转录病毒 ,并将病毒感染L9981细胞 ,获得L9981 nm 2 3 H1 。应用PCR和Westernblot检测L9981 nm2 3 H1 细胞中nm2 3 H1基因及其蛋白表达。结果 成功构建了逆转录病毒载体pLXSN nm2 3 H1 EGFP。nm2 3 H1 cDNA导入到L9981细胞株中 ,并检测到L9981 nm 2 3 H1 细胞中有nm 2 3 H1 蛋白表达。结论 nm 2 3 H1 基因在细胞株L9981 nm2 3 H1 中能持续。
Objective To establish a human large cell lung cancer cell line L9981 nm23 H1 transfected with wild type nm23 H1 gene. Methods pLXSN nm23 H1 EGFP was constructed by gene clone technique, and L9981 nm23 H1 was established by infected virus of nm23 H1 gene. The DNA and protein expression of nm23 H1 were detected in the transgene large cell lung cancer cell line by PCR and Western blot. Results pLXSN nm23 H1 EGFP was constructed successfully, and the nm23 H1 cDNA was inducted to L9981 cell. The protein of nm23 H1 could be detected in L9981 nm23 H1 cell. Conclusion Protein of nm23 H1 is stably, continuously and high efficiently expressed in L9981-nm23-H1 cell.
出处
《中国肺癌杂志》
CAS
2004年第3期187-190,共4页
Chinese Journal of Lung Cancer
基金
国家自然科学基金 (No .30 0 70 333和No.30 1 0 0 0 75)资助~~
