摘要
目的研究DNA结合/分化抑制蛋白-1(Id-1)在结直肠癌(CRC)中的表达,并通过分析其与碱性成纤维细胞生长因子(bFGF)、增殖细胞核抗原(PCNA)、Bcl-2的关系,探讨其在CRC发生发展过程中的意义。方法收集CRC手术切除标本103例,癌旁结直肠组织20例(离癌灶<3 cm),并以20例正常结直肠组织为对照,免疫组织化学方法检测其Id-1、bFGF、Bcl-2和PCNA的表达。结果 CRC中Id-1、bFGF、Bcl-2和PCNA的阳性表达率分别为80.6%(83/103)、78.6%(81/103)、70.9%(73/103)、81.6%(84/103),其阳性表达率和表达强度均明显高于正常结直肠组织。Id-1及bFGF的表达与结直肠癌的分化程度呈正相关。Id-1在≥5 cm的CRC中的表达高于<5 cm的CRC。Id-1的表达与CRC的淋巴结转移无相关性。Id-1的表达与bFGF、Bcl-2及PCNA呈正相关性(分别为r=0.372,P<0.001;r=0.274,P=0.005;r=0.303,P=0.002)。结论 Id-1在CRC中可能参与了肿瘤血管形成、抑制细胞凋亡的调控及细胞增殖调控,但可能不直接参与CRC的转移。
Objective To study the relationship between the expression of inhibitor of differentiation/ DNA binding-1（Id-1） and the expressions of basic fibroblast growth factor（bFGF）,Bcl-2 and proliferating cell nuclear antigen（PCNA）,and the potential role of Id-1 in the carcinogenesis of colorectal cancer（CRC）.Methods A total of 103 cases of CRC specimens and 20 cases of adjacent non-tumor colorectal tissue were obtained,and 20 cases of normal colorectal tissue specimens were used for comparison.Immunohistochemical technique was applied to detect the expressions of Id-1,bFGF,Bcl-2 and PCNA.Results The positive expression rates of Id-1,bFGF,Bcl-2 and PCNA in tumor tissues were 80.6%,78.6%,70.9% and 81.6%,respectively.The positive expression rate and intensity were significantly higher than those in normal colorectal tissues.The expression of both Id-1 and bFGF was positively correlated with the histological grade.The positive rate of Id-1 was significantly correlated with the tumor size and was not correlated with the tumor metastasis.The expression of Id-1 was positively associated with bFGF,Bcl-2 and PCNA.Conclusion The expression of Id-1 may be involved in the vascularization of CRC,the regulation of apoptosis and proliferation of CRC cells.The results of the current study suggest that the increased expression of Id-1 may play an important role in CRC size but the expression of Id-1 is not associated with tumor metastasis.
出处
《福建医科大学学报》
2011年第1期50-53,共4页
Journal of Fujian Medical University
关键词
结直肠肿瘤
分化抑制蛋白1
成纤维细胞生长因子2
增殖细胞核抗原
免疫组织化学
Colorectal Neoplasms
Inhibitor of Differentiation Protein 1
Fibroblast Growth Factor 2
Proliferating Cell Nuclear Antigen
Immunohistochemistry
