摘要
The studies from 7 or 8 days old mice were used to prepare the spermatogonial stem cells.The isolation and purification of spermatogonial stem cells were done by means of the Percoll discontinual density gradient concentration.The cells of the 3rd band were collected and cultured in vitro in DMEM supplemented with 2.5%or 10%fetal bovine serum(FBS).The results showed that with the feeder layer and 2.5%serum,the spermatogonial stem cells could proliferate,differentiate last more than 4 months.
