摘要
收集新生牛睾丸,进行组织学观察与组织的体外培养,在10%血清培养液中培养1周和2周后进行组织学观察与RT-PCR分析。结果表明:新生牛睾丸组织经体外培养1周时,管腔直径增大,精原干细胞数明显增多;体外培养2周时,管腔直径继续扩增,精原干细胞数量减少;在新鲜睾丸组织、培养1周和2周的睾丸组织中PGP 9.5持续表达。
Testes were collected from neonatal bull for histological observation and in vitro culture.Testicular tissues in medium supplemented with 10% serum were cultured for 1 week and 2 weeks later for histological observation and RT-PCR analysis.The results showed that at 1 week after culture,seminiferous tubule diameter and the number of spermatogonia were increased significantly;at 2 week after culture,seminiferous tubule diameter continued to increased and the number of spermatogonia was decreased;testicular tissues of fresh,1,and 2week-cultured tissues continued to express PGP9.5.
出处
《中国畜牧杂志》
CAS
北大核心
2010年第15期22-24,共3页
Chinese Journal of Animal Science
基金
黑龙江省农业科学院博士后基金资助(LRB04-185)
关键词
睾丸
体外培养
组织学
牛
testis
in vitro culture
histology
bull
