摘要
为了探究外源NO浸种对盐胁迫下沙葱种子萌发及抗氧化物酶系活性的影响,以沙葱种子为材料,观察不同浓度和浸种时间的外源NO处理对NaCl胁迫下沙葱种子萌发、子叶、胚根长度及干质量、抗氧化酶系和MDA变化的影响。研究结果表明,经0.40 mmol/L SNP浸种3 h能显著(P<0.05)提高沙葱种子在NaCl胁迫下的萌发率、萌发速率、萌发指数、活力指数、以及子叶和胚根的长度和干质量;该浸种处理能有效地提高NaCl胁迫下种子内SOD、CAT、POD和APX抗氧化酶的活性,从而增强种子萌发抗NaCl胁迫的能力;NaCl胁迫处理促进了沙葱种子细胞的膜脂过氧化,使其MDA含量提高;但0.40 mmol/L外源NO处理明显缓解了这种氧化损伤,降低了种子的膜脂过氧化作用。
In order to investigate the effects of seed soaking with exogenous NO on seed germination,activities of antioxidant enzymes of Allium mongolicum Regel.under NaCl stress.The experiment used the seed of A.mongolicum as experiment material to explore the effect of different concentrations of NO soaking different times on A.mongolicum seed germination,hypocotyl,radicle length and dry weight,as well as the effect of SOD,CAT,POD,APX and content of MDA.In our study,the soaked seeds with 0.40 mmol/L NO for 3 hours could significantly（P0.05）enhance the germination percentage,germination rate,germination index,vigor index,length and dry weight of cotyledon and radicle,meanwhile this treatment could increase the activitvies of antioxidant enzymes：SOD,CAT,POD,APX,additionally under NaCl stress,lipid proxidation of the seed of A.mongolicum was promoted,therefore the content of MDA was increased,but oxidative damage of seed is relieved under the treatment of 0.40 mmol/L exogenous NO and lipid proxidation was reduced.
出处
《华北农学报》
CSCD
北大核心
2011年第2期207-212,共6页
Acta Agriculturae Boreali-Sinica
关键词
沙葱
种子萌发
NACL胁迫
抗氧化物酶
外源NO
Allium mongolicum Regel
Seed germination
NaCl stress
Antioxidant enzymes
Exogenous NO
