摘要
目的 :用免疫组织化学方法观察周围神经损伤后的结构改变及时相。方法 :切断SD大鼠的双侧坐骨神经 ,用免疫组织化学ABC法 ,SMI 3 1轴索染色及S 10 0雪旺细胞染色观察 6h到 4周神经远端的结构变化。结果 :与以往组织学观察所见不同的是 ,神经损伤平面近端 10mm范围内 ,1周内存在酶组织化学损伤性反应 ,6h起出现轴索的形态学改变。神经损伤平面远端全长 1～ 3周形成密集排列的细胞条索 ,但S 10 0染色阴性。结论 :采用免疫组织化学的方法能够更早的观察到神经变性的发生 ,神经损伤近端观察到更广泛的变化 ,S -10
To observe the structural change and phase of injured peripheral nerve by immunohistochemical stain.Methods:The structural change in proximal and distal stump of sciatic nerve of SD rats from 6 hours to 4 weeks post transected were studied by immunohistochemical method.Antibody to SMI 31 and S 100 was used to stain the axon and Schwann′s cells respectively.Results:Different from previous report,an enzymical reaction within 10 mm of the proximal end of the transected nerve was found in one week after injury,and the morphological change of axons could be found 6 hours after injury.High density of cell bunch was formed after 1 to 3 weeks of injury in the distal stump,while S 100 stain was negative in Schwann′s cell.Conclusion:Phase of nerve degeneration and change in the proximal end of transected nerve could be found earlier by immunohistochemical study after injury.S 100 may be an important marker for normal or activated Schwann′s cell.
出处
《中国临床解剖学杂志》
CSCD
北大核心
1999年第4期353-355,共3页
Chinese Journal of Clinical Anatomy
关键词
周围神经变性
轴索
雪旺细胞
免疫组织化学
Peripheral nerve Axon Schwann′s cell Degeneration I mmunohistaochemical
