摘要
目的探讨加热(hyperthermia,HT)联合紫杉醇(Taxol)对人喉癌细胞系(Hep-2细胞)体外增殖抑制及凋亡的影响。方法将Hep-2细胞分为实验组与对照组,实验组用不同浓度(0.1、1.0及10.0μmol/L)的Taxol预处理后联合热疗(39、41及43℃1 h)处理不同时间(24、48及72 h)后,采用Wright-Gimsa染色法观察Hep-2细胞凋亡的形态学变化;以四甲基偶氮唑盐(MTT)还原试验检测细胞活力,以细胞增殖率作为细胞损伤指标,流式细胞术检测细胞凋亡发生率。结果加热41℃组细胞增殖率与其他不同温度同剂量组比较均显著降低(P<0.01);39℃组与对照组37℃组比较差异无统计学意义(P>0.05);热疗联合Taxol组处理细胞48 h后,细胞出现凋亡形态学改变。与热疗和Taxol单药组相比,热疗联合Taxol组对细胞的抑制率显著增强(P<0.01),其作用呈时间和剂量依赖性;热疗联合Taxol组诱导细胞凋亡率与单药组及单热疗组相比较均增高(P<0.05);而单药紫杉醇组与单独热疗组之间比较差异无统计学意义(P>0.05)。结论热疗和Taxol单药体外均可抑制Hep-2细胞增殖并诱导其调亡。热疗和Taxol联合应用对Hep-2细胞体外增殖抑制其诱导凋亡作用显著增强;诱导凋亡可能是细胞增殖抑制的作用机制之一。
【Objective】To determine the effect of hyperthermia combined with Taxol on the proliferation and apoptosis of human larynx carcinoma Hep-2 cells in vitro.【Methods】Hep-2 cells were divided into an experimental group and a control group,and the experimental group was further divided into 0.1 μmol/L Taxol group,1.0 μmol/L Taxol group,10 μmol/L Taxol group and hyperthermia group,Hep-2 cells were treated with hyperthermia combined with Taxol Wright-Giemsa dying assay was used to observe the apoptosis morphology of Hep-2 cells.The proliferation of the cells treated with hyperthermia and Taxol was detected by the method of 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT）,colony formation test and Flow cy-tometry（FCM） was used to detect the apoptosis rate of lymphoma and the fluorescene density in the lympho-cytes.【Results】Compared with the same dosage of paclitaxel but different temperature,the Hep-2 cell prolif-eration rate in the group heating at 41℃ decreased significantly（P 0.05）.The proliferation rate of Hep-2 cell in 39℃ and 43℃ groups decreased significantly compared with the 37℃ control group（P 0.05）.Evident apoptosis morphological changes of Hep-2 cells were observed 48 hours ofter treatment with hyperthermia or Taxol,compared with hyperthermia or Taxol alone,hyperthermia combined with Taxol could increase the inhi-bition ratio sisnificantly（P 0.01）,and the inhibition rate was related to the concentration and action time of Taxol（P 0.05）,compared with hyperthermia or Taxol alone,hyperthermia combined with Taxol could increase the apoptosis rate Hep-2 cells with obvious difference（P 0.05）.Conclusion of The reverse effect of hyper-thermia combined with Taxol 48 h on Hep-2 cells was determined by cell growth curve,the doubling time and the number of colony formation were calculated based test.Light and electronic microscopy was used to observe morphological changes of Hep-2 cell lines.The apoptotic rate of Hep-2 cell lines in both testing group and control group cells were analyzed by flow cytometry.The cells apoptosis rates were detected by flow cytometry（FCM）.Result compared with Taxol or hyperthermia,the hyperthermiaat 41℃ for 1 hour com-bined with Taxol showed obvious inhibitory effect in Hep-2 cells（P 0.05）.【Conclusion】Hyperthermia or Taxol alone or combined can inhibit the proliferation,induce cell apoptosis,hyperthermia combined with Taxol has synergistic anti-Hep-2 cells effect in vitro.Hyperthermia combined with Taxol can inhibit the prolifera-tion and apoptosis of Hep-2 cells in vitro and inhibit proliferation in vitro with synergistic effect.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第11期1311-1316,共6页
China Journal of Modern Medicine
基金
国家自然科学基金青年科学基金(No:30901664)
关键词
人喉癌细胞系
热疗
紫杉醇
凋亡
化疗敏感性
carcinoma of larynx cell line
hyperthermia
taxol
apoptosis
chemosensitivily
