摘要
目的探讨Twist、N-cadherin蛋白在喉鳞状细胞癌(喉鳞癌)组织中的表达及临床意义。方法选择喉鳞癌标本80例,同时选择癌旁组织标本30例,应用免疫组化SP法检测Twist、N-cadherin蛋白表达情况。结果Twist、N-cadherin蛋白在喉鳞癌组织中的阳性表达率高于癌旁组织,差异均有统计学意义(P<0.01);Twist、N-cadherin蛋白的表达在低度分化、Ⅲ～Ⅳ期、有淋巴结转移和有远处转移的喉鳞癌组织中分别明显高于高、中度分化、Ⅰ～Ⅱ期、无淋巴结转移和无远处转移的喉鳞癌组织(P<0.05,P<0.01),而与性别、年龄无关(P>0.05);Twist与N-cad-herin蛋白在喉鳞癌组织中的表达呈明显正相关(r=0.402,P<0.01)。结论 Twist、N-cadherin蛋白高表达可作为喉鳞癌发生浸润转移的重要生物学指标,预示肿瘤的侵袭性较高,预后不良。
Objective To investigate the expression of Twist protein and N-cadherin protein in laryngeal squamous cell carcinoma and clinical significance.Methods Application immunohistochemical SP methods to detect Twist protein and N-cadherin protein in 80 cases of laryngeal squamous cell carcinoma.Meanwhile,30 samples of adjacent tissue to cancer were also evaluated.Results Expression of Twist protein and N-cadherin protein in laryngeal squamous cell carcinoma were significantly higher than that in adjacent cancer tissue（P0.01）.The expression of Twist protein and N-cadherin protein in samples of low differentiation stages Ⅲ-Ⅳ,and in metastatic sites such as lymph nodes and distant organs were significantly higher than that in samples of highly,moderately differentiation stages of Ⅰ-Ⅱ,and normal lymph nodes and normal distant organs（P0.05,P0.01）,which was not associated with gender or age（P0.05）.A positive correlation was found between Twist protein and N-cadherin protein in laryngeal squamous cell carcinoma（r=0.402,P0.01）.Conclusion The overexpression of Twist protein and N-cadherin protein might be an important biological marker for invasion and metastasis of laryngeal squamous cell carcinoma,indicative of high invasiveness of this cancer and poor prognosis.
出处
《解放军医药杂志》
CAS
2012年第4期17-19,共3页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
