摘要
研究以140只京海黄鸡为试验材料,以MC4R为候选基因,采用PCR-SSCP和DNA测序技术分析了MC4R基因在京海黄鸡群体中的多态性。结果表明:MC4R基因编辑区第662bp处有G→C碱基的点突变,在京海黄鸡中检测到AA、AB、BB3种基因型,A等位基因的频率为0.929,B等位基因的频率为0.071。经过基因型与生长性能的关联分析得知:不同基因型与京海黄鸡4、8、12周龄体重差异显著(P<0.05),与16周龄体重差异极显著(P<0.01)。由此推测,MC4R基因可能对于鸡生长性能具有很大的影响或与控制生长性能的主基因连锁。
In this study,Jinghai Yellow chicken was used to analyze MC4R gene polymorphisms by PCR-SSCP and DNA sequencing method. The result indicated that there was one mutation site in Jinghai Yellow Chicken：G662C. Three genotypes （AA,AB,BB） were detected,and the frequencies of allele A and B were 0.957 and 0.982 respectively. The genotypes showed a significant difference in 4-week-age body weight,8-week-age body weight,12-week-age body weight （P〈0.05）. It implied that MC4R gene could have a major effect on growth performance or could be linked to gene （s）that significantly affect growth performance in chicken.
出处
《中国家禽》
北大核心
2008年第5期21-23,共3页
China Poultry
基金
江苏省高新技术项目(BG2004316)
江苏省“青蓝工程”培养基金
