摘要
目的 比较第二代和第三代丙肝试剂对丙肝不同功能区抗体的检出能力。方法 用两种第二代抗 Ｈ Ｃ Ｖ Ｅ Ｉ Ａ 试剂（ Ｋ２ 中国制造， Ａ２ 美国制造） 及两种第三代主流试剂（ Ａ３ 德国制造， Ｏ３ 美国制造） 对１２１ 份来源于全国各地的供血员血样进行了检测。结果 该１２１ 份样品经 Ｒ Ｉ Ｂ Ａ Ｈ Ｃ Ｖ３ ．０ 试剂及 Ｐ Ｃ Ｒ 方法确证， 其中４３ 份为阳性样品，７８ 份为阴性样品。两种第二代试剂 Ｋ２ ， Ａ２阳性检出符合率为３６／４３ 和３７／４３ ， 两种第三代试剂 Ａ３ ， Ｏ３ 阳性检出符合率分别为４０／４３ 和３９／４３ 。在４３ 份阳性样品中，２０ 份既含有抗 Ｈ Ｃ Ｖ 核心抗体， 也含有抗 Ｈ Ｃ Ｖ Ｎ Ｓ３ 抗体，用 Ｋ２ ， Ａ２ ， Ａ３ ， Ｏ３ 四种试剂分别检出１８ 、１８ 、１９ 和２０ 份。１４ 份含有抗 Ｈ Ｃ Ｖ Ｎ Ｓ３ 抗体而不含有抗 Ｈ Ｃ Ｖ 核心抗体的样品中，用两种第二代试剂（ Ｋ２ ， Ａ２） 分别检出９ 份和１０ 份， 而用两种第三代试剂（ Ａ３ ， Ｏ３） 则全部检出。与此相反，９ 份含有抗 Ｈ Ｃ Ｖ 核心抗体而不含抗 Ｈ Ｃ Ｖ Ｎ Ｓ３ 抗体的样品， 用两种第二代试剂（ Ｋ２ ， Ａ２）均可检出， 而用两种第三代试剂（ Ａ３ ， Ｏ３）
Evaluation on the capabilities for detecting antiHCV core and NS3 antibodies with two antiHCV EIA kits of the third generation$$$$ QI Zibai, ZHOU Cheng, YANG Zhen, et al. National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050 【 Abstract 】 Objective To evaluate the capabilities for detecting antiHCV core and NS3 antibodies with the third generation antiHCV EIA kits. Methods Forty three weak positive and 78 negative antiHCV sera were collected from Chinese blood donors. Four commercial antiHCV EIA kits, two kits (K2,A2) of second generation and two kits (A3, O3) of third generation were used to detect these samples. All the sera were confirmed with Chiron RIBA HCV 3.0 and PCR. Results All 43 positive sera contained HCV RNA. Among 43 samples, 20 were both antiHCV core and NS3 positive detected with O3 and 18, 19 and 19 were so detected respectively with K2, A2 and A3. Fourteen samples were anticore negative and NS3 positive detected with A3 and O3, while 9 and 10 samples were so detected respectively with K2 and A2. Nine samples were anticore positive and NS3 negative detected with K2 and A2, while 7 with A3 and 5 with O3 were so. Seventy eight negative samples did not reacted with A3 and O3, but 4 false positive for K2 and 2 false positive for A2 were found. Conclusions The results showed that the third generation antiHCV EIA kit can detect more HCV positive sera than the second one (A3, 40; O3, 39; Vs K2, 36; A2. 37). But the capability for detecting antiHCV core antibody by the third generation antiHCV EIA kits still need to be improved. 【 Subject words 】 Hepatitis C HCV core antibody HCV NS3 antibody
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1999年第5期433-435,共3页
Chinese Journal of Microbiology and Immunology
基金
"九五"国家科技攻关课题
