摘要
目的:研究胆囊收缩素(cholecystokinin,CCK)rs747455位点及胆囊收缩素A受体(cholecystokinin A receptor,CCKAR )rs1800856位点的基因多态性与上海市区人群胆道癌及胆石症易感性的关系。方法:采用全人群病例-对照研究的方法,运用实时荧光定量PCR系统对253例胆囊癌、133例肝外胆管癌、53例壶腹癌和440例胆石症患者以及445名正常对照进行CCK rs747455及CCKAR rs1800856位点基因型分析。结果:无胆石个体中携带CCK rs747455位点CT基因型者较携带CC基因型者罹患壶腹癌的风险降低,其比值比(odds ratio,OR)=0.31,95%可信区间(confidence interval,CI)为0.10～0.96,经过Bonferroni校正后此差异仍有统计学意义(P=0.042);饮酒个体中携带rs747455位点TT基因型或CT基因型者较携带CC基因型者罹患胆石症的概率增加(OR=2.81, 95%CI:1.08～7.29;OR=2.93, 95%CI:1.03～8.33),但经过Bonferroni校正后差异无统计学意义(P=0.061)。在CCKAR rs1800856位点的分层分析中未发现该位点与胆道癌及胆石症有显著相关性。结论:CCK基因rs747455位点可能与中国上海人群胆道癌及胆石症的发生相关。
Objective：To investigate the relationship between the gene polymorphisms of cholecystokinin（CCK） and cholecystokinin A receptor（CCKAR） and genetic susceptibilities of biliary tract cancer and cholelithiasis in a Chinese population from Shanghai.Methods：A population-based case-control study was carried out in 253 cases of gallbladder cancer,133 cases of extrahepatic bile duct cancer,53 cases of cancer of Vater＇s ampulla,440 cases of biliary stones,and 445 population controls.The genotypings of CCK rs747455 and CCKAR rs1800856 were performed by Real-time PCR.Results：Non-stone cases carrying the CCK rs747455 CT genotype reduced the risk of ampulla cancer,compared with the carriers of CC genotype [odds ratio（OR）=0.31,95% confidence interval（CI）=0.10-0.96],and this association was still significant after Bonferroni correction（P=0.042）.The drinkers who carrying TT or CT genotype increased the risk of cholelithiasis,compared with the carriers of CC genotype（OR=2.81,95% CI=1.08-7.29;OR=2.93,95% CI=1.03-8.33）,but this association was not significant after Bonferroni correction（P=0.061）.The genotyping of CCKAR rs1800856 was not associated with the risk of biliary tract cancer and cholelithiasis.Conclusion：CCK rs74745 gene polymorphism may be correlated to the susceptibilities of biliary tract cancer and cholelithiasis in a Chinese population form Shanghai,China.
出处
《肿瘤》
CAS
CSCD
北大核心
2011年第3期239-244,共6页
Tumor
关键词
胆道肿瘤
胆石
胆囊收缩素
受体
胆囊收缩素A
多态性
单核苷酸
Biliary tract neoplasms
Gallstones
Cholecystokinin
Receptor
cholecystokinin A
Polymorphism
single nucleotide
