摘要
应用间接免疫荧光法和显微荧光分光光度技术，研究了试管内无细胞毒性浓度的亚硒酸纳（硒）对来源于伯基特淋巴瘤的Ｒａｊｉ细胞株，ＥｐｓｔｅｉｎＢａｒ病毒（ＥＢＶ）抗原表达的影响以及抑制ＥＢＶ转化人脐血Ｂ淋巴细胞（ＢＬ）的作用。结果表明，００１～０１ｍｇ／Ｌ的硒使Ｒａｊｉ细胞的ＥＡ抗原抑制３５３％～５０１％；００１～０５ｍｇ／Ｌ的硒令ＥＢＮＡＭＦＳ值降低８４％～２１８％；０１～１０％ｍｇ／Ｌ的硒明显抑制ＥＢＶ转化ＢＬ，抑制率为６３３％～９２１％。由于ＥＢＶ与鼻咽癌（ＮＰＣ）密切相关，ＮＰＣ病人血清和头发中硒含量明显低于健康人。本研究提示：对ＮＰＣ患者和ＥＢＶ基因表达活跃的人群适当补硒，可能有助于ＮＰＣ和与ＥＢＶ相关疾病的预防和辅助治疗。
The effects of non toxic doses of sodium selenite(Se)on the expression of Epstcin Barr virus (EBV) antigens in Raji cell line originated from Burkitt's lymphoma were studied by indirect immunofluorescence staining and micro fluorescence spectrophoto quantitation (MFS),as well as the inhibiting transformation in B lymphocytes from human umbilical cord blood (BL) with EBV in vitro by Se. It was found that the early antigen (EA)was inhibited 35.3%～50 1% by Se (0.01～0.1 mg/L) and Se (0.01～0.5mg/L)can lower nnclear antigen (EBNA) MFS value for 8.4%～21.8% in Raji cell linc.The transformation effect of BL with EBV was inhibited by Se (0.1～1.0mg/L).The inhibition rate of BL transformation was 60.3%～92.1%.EBV is associated with the nasopharyngeal carcinoma(NPC).The Se levels in serum and hair of patients with NPC were lower than that of healthy persons.The results suggest that supplement Se to patients with NPC and persons for active expression of EBV gene may protect against and treat NPC and diseases associated with EBV.
出处
《广东微量元素科学》
CAS
1999年第6期12-15,共4页
Trace Elements Science
关键词
硒
癌细胞株
B细胞
抗原表达
EB病毒效应
Selenium,Human cancer cellular line,Epstein Barr virus (EBV),Antigen expression,Lymphocyte transformation
