摘要
目的：观察γ射线诱导鼻咽癌细胞凋亡情况。方法：显微荧光染色法和原位末端标记法观察放射线对人鼻咽癌细胞株（ＨＮＥ１）凋亡情况进行研究。结果：γ射线０、３、６、９、１２、２５Ｇｙ照射后ＨＮＥ１细胞凋亡率荧光法分别为５．５％、９．５％、１２％、２５．５％、３５．５％、６５．５％；末端标记法分别为１．５％、１１％、１５．５％、２１．５％、２６．５％及５０．５％。结论：放射诱导凋亡在ＨＮＥ１放射效应中起重要作用，其凋亡率随照射剂量增加明显提高。
Objective: To investigate the effect of radiationinduced apoptosis in nasopharygeal carcinoma cells. Methods: Nasopharygeal carcinoma cell lines (HNE1) in vitro were observed with the fluorescence microscopy (FM) and in situ end labeling (ISEL) after gamma radiation. Results: After the irradiation of 0, 3, 6, 9, 12 and 25Gy, AP rates of HNE1 were 5.5%, 9.5%, 12%, 25.5%, 35.5% and 65.5% respectively in FM and 1.5%, 11%, 15.5%, 21.5%, 26.5% and 50.5% respectively in ISEL. Conclusion: Radiationinduced apoptosis plays an important role in HNE1 radiation response. AP rates of HNE1 enhance significantly with the increase of radiation doses.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
1999年第2期100-102,共3页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
湖南省卫生厅基金
CMB基金
关键词
放射线
鼻咽肿瘤
细胞凋亡
实验研究
RadiationCarcinoma, nasopharyngeal neoplasmApoptosis
