摘要
人胎脑经离心、透析和离子交换纤维素层析等方法，分离纯化２．５Ｓ神经生长因子（ＮＧＦ）。根据７ＳＮＧＦ和其活性亚单位β－ＮＧＦ的等电点的不同，分离得到２．５Ｓ的ＮＧＦ。用ＳＤＳ－聚丙烯酰胺电脉测得其分子量约１３．３。用等电聚焦电泳测得其等电点分别为９．０、９．２和９．３５。在ＰＣ１２细胞的培养过程中，加入ＮＧＦ的提取物可以促进ＰＣ１２细胞的突起生长。
Nerve growth factor (2.5S NGF) was seperated from
human brain by centrifugation,dialyzing and ionexchange chromatography.Then 2.5S NGF was
isolated by CM Cellulose52 chromatography based on the difference of the purified NGF in
isoelectric points between 2.5S NGF and 7S NGF.The isoeletric points of the three components
NGF were 9.0,9.2,9.35 respectively detected by isoelectrifocusing electrophoresis.The
molecular weight of 2.5S NGF detected by SDSPAGE was about 13.3. PC12 cell culture reveals
that the purified NGF shows the effect of stimulating neurite growth.
出处
《苏州医学院学报》
1999年第7期761-762,810,共3页
Acta Academiae Medicinae Suzhou
关键词
神经生长因子
分离
纯化
生物学活性
鉴定
nerve growth
factor
isolation
purification
biological activity
