摘要
胶质细胞成熟因子(GMF)是从成年牛脑中提取的一种酸性蛋白质,它能可逆地促进成星形胶质细胞形态和化学的分化。我们现在建立富含神经元的生后7天大鼠小脑皮质分离细胞原代培养物,间接免疫荧光染色分类计数证明,该原代培养物中神经元占细胞总数的97%,其中绝大部分是颗粒细胞。加纯化的 GMF 在此培养物,可明显促进神经元的生存;而不加 GMF 的对照培养物,神经元的数目明显减少。纯化 GMF 的这种作用与其剂量有关,最适的刺激剂量浓度为250 ng/ml。虽然对 GMF 影响小脑皮质神经元生存的机制尚不清楚,但实验结果提示,GMF 的功能不只限于对胶质细胞,它在中枢神经系统内,也可能是一种对神经元起神经营养作用的生长因子。
Glia maturation factor(GMF)detected in the adult brain is an acidic protein
that has the ability to promote the morphological and chemical differentiation of
astroblasts,but this effect is reversible.In the present study we have established
neuron-enriched dissociated primary cultures from 7-day-old rat cerebellar cortex
in which about 97% of the cells are neurons especially granule cells using differential count of indirect immunofluorescence.The addition of purified GMF to the cultures
markedly enhances neuronal survival,while control cultures grown in the absence of
GMF exhibit a significant decrease in neuronal number.These GMF effects are
dose-dependent,with optimal stimulation occurring at a concentration of 250 ng/ml.
Although the mechanism by which purified GMF influences cerebellar cortical
neuronal survival is not known,these results suggest that GMF not only affects
glial cells,but also may function as a neurotrophic agent in the central nervous
metsys
出处
《解剖学报》
CAS
CSCD
北大核心
1989年第1期50-55,共6页
Acta Anatomica Sinica
关键词
GMF
原代培养
小脑
神经元
大鼠
Survival of cerebellar cortical neurons
Primary culture
Indirect immunofluorescence staining
Rats
