摘要
目的探讨五味保肝丸对大鼠急性四氯化碳(CCl4)肝损伤的保护作用。方法将60只Wistar大鼠随机分成6组,每组10只。正常对照组、模型对照组灌胃给予20 g/L羧甲基纤维素钠溶液,联苯双酯组灌胃给予10 g/L联苯双酯混悬液,五味保肝丸高、中、低3个剂量组分别灌胃给予0.315 00、.157 5和0.078 8 kg/L五味保肝丸混悬液,各组给药量均为20 mL/kg,每日灌胃1次,连续7 d。末次给药2 h后,除正常对照组大鼠腹腔注射等量生理盐水,其余5组均腹腔注射CCl4原液1 mL/kg。检测各组大鼠血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,肝组织匀浆中超氧化物歧化酶(SOD)、丙二醛(MDA)含量,观察肝脏组织病理学变化情况。结果与模型对照组比较,五味保肝丸高、中剂量组血清中ALT活性降低,肝组织匀浆MDA含量降低,差异均有显著性(F=3.883、6.943,q=3.578～4.553,P<0.05);但AST、SOD的含量差异无显著性(P>0.05)。结论五味保肝丸对急性CCl4肝损伤大鼠的肝组织具有保护作用。
Objective To investigate the protection of Wuweibaoganwan（WWBGW）-traditional Chinese medicine-onacute hepatic damage in rats caused by carbon tetrachloride（CCl4）. Methods Sixty Wistar rats were evenly randomized to six groups： normal control（NC）,model control（MC） group,bifendate group,high-,medium-,and low-dose WWBGW group.To those in NC and MC group,20 g/L CMC-Na solution was given;those in bifendate group,treated with 10 g/L bifendate suspl;those in high-,medium-,and low-dose WWBGW group,were treated with WWBGW suspl.of 0.315 0,0.157 5,and0.078 8 kg/L,respectively.The medication in all groups was given through intragastric administration,once a day for seven days.After 24 h of the last medication,CCl4（1 mL/kg） was given to those in the five groups,except NC group,by way of intraperitoneal injection,and to those in NC group,equal dose of normal saline was injected.The activity of serum GPT and GOT;the contents of superoxide dismutase（SOD） and malondialdehyde（MDA） in homogenate of liver as well as pathologic changes of liver tissue were observed. Results WWBGW notably decreased the activity of serum ALT,AST and liver MDA,and increased SOD in model rats. Conclusion WWBGW possesses a significant protection effect on liver and decreases aminopherase in model rats with acute hepatic damage induced by CCl4.
出处
《齐鲁医学杂志》
2011年第3期219-221,224,共4页
Medical Journal of Qilu
基金
青岛市科技局资助项目(06-2-2-6-nsh)
