摘要
基于在十二烷基硫酸钠存在下的弱碱性介质中Fe(Ⅲ)对H_2O_2氧化2,4-二氯苯酚(2,4-DCP)与4-氨基安替比林(4-AAP)偶联显色反应的催化作用,建立了检出限为7.90μg/L.线性范围为0～1.25mg/L的新催化光度分析法.方法用于血红细胞中铁的测定,相对标准偏差与回收率分别为2.6%与98.8%～101%,结果满意.
The catalytic coupling reaction of 2,4-dichlorophenol and 4-amino antipyrine with hydrogenperoxide by iron(Ⅲ) in a weak base medium was studied and its optimum conditions were established .The absorbance of the red product was measured at 502 nm with a detection hrnit of 7.9μg/L. The linearrange of this method was 0～1.25 mg/L and the relative standard deviation was 2.6%. The proposedmethod was used to determine trace iron in erythrocyte with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第12期1397-1400,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.29975016)
山东省杰出青年科学家基金(No.98015)
山东省自然科学基金(No.97B13036)
关键词
铁
分光光度法
催化动力学
偶联反应
血红细胞
Iron
spectrophotometry
catalytic kinetics
coupling reaction
erythrocyte
