摘要
目的探讨人肝细胞癌组织中干细胞标志物ABCG2的表达及临床意义。方法采用实时荧光定量PCR和免疫组织化学方法检测40例肝细胞癌及癌旁组织中ABCG2 mRNA和蛋白的表达。结果 ABCG2mRNA在肝细胞癌组织中的表达是癌旁组织的1.619倍,差异有显著性(P=0.000)。肝细胞癌及癌旁组织中ABCG2蛋白阳性表达率分别为47.5%(19/40)、22.5%(9/40),两组比较差异有显著性(P=0.019);ABCG2蛋白的表达与患者的性别、年龄、肿瘤大小等均无关(均P>0.05),而与肝癌分化程度、肝癌包膜是否受累及是否有癌栓关系密切(均P<0.05)。结论 ABCG2在肝细胞癌组织中的表达明显高于癌旁组织,且与组织分化程度、肝癌包膜是否受累及是否有癌栓等临床病理特征有关,提示ABCG2在肝细胞癌的发生、发展过程中发挥一定作用,可能与肝细胞癌的侵袭和转移有关。
【Objective】 To investigate the expression and significance of ABCG2 in tissues of hepatocellular carcinoma（HCC）.【Methods】 Real-time fluorescence quantitative PCR and immunohistochemistry Envision methods were utilized to detect the expression of ABCG2 in 40 cases of HCC tissues and matching tumor-adjacent tissues.【Results】 The quantity of mRNA in HCC tissues is 1.619 times as much as that in tumor-adjacent tissues.Positive rate of ABCG2 protein in HCC tissues was 47.5%,which was significantly higher than 22.5% in tumor-adjacent tissues（P 0.05）.The expression of ABCG2 protein was not concerned with gender,age or tumor size,but was significantly correlated with poorly-differentiation,PVTT and capsule violation（P 0.05）.【Conclusion】 Over expression of ABCG2 in HCC tissues may participate in the carcinogenesis and development of HCC.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第8期976-980,共5页
China Journal of Modern Medicine
基金
河南省科技厅基础与前沿研究项目(No:082300450100)
