摘要
目的:比较21个不同种源薏苡仁中甘油三油酸酯含量。方法:采用HPLC-ELSD法,色谱柱为Discovery C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-二氯甲烷(66∶34),流速为0.7 mL.min-1,柱温为30℃;采用蒸发光散射检测器,漂移管温度为45℃;氮气流速为1.5 L.min-1。结果:21个不同种源的薏苡仁中甘油三油酸酯含量都符合《中国药典》标准,含量最高是种子来自福建三明宁化的粳性薏苡仁,为0.793%;含量最低是种子来自浙江缙云的薏苡仁,为0.561%。结论:21个不同种源的薏苡仁中甘油三油酸酯含量有一些差异,种子来自同一地区的薏苡仁中甘油三油酸酯含量也有一些差异。
Objective： To compare the content of glycerol trioleate in 21 Semen Coicis of different provenances. Methods： The Discovery C18 column （250 mm × 4.6 mm, 5 μm） was used with mobile phase of acetonitrile-dichloromethanol（66：34）. The mobile phase flow rate was 0. 7 mL·min^-1 The column temperature was controlled at 30 ℃. The ELSD was used. The tube temperature was 45 ℃, and the flow rate of nitrogen was 1.5 L·min^-1 Results： The content of glycerol trioleate in 21 semen coicis of different provenances were in line with Chinese pharmacopeia, and the highest content was japonica Semen Coicis which seeds from Ninghua, Sanming and Fujiang, which was 0. 793 % ; the lowest content was the sample which seeds from Jinyun and Zhejiang, which was 0. 561%. Conclusion： The content of glycerol trioleate in 21 Semen Coicis of dirrerent provenances is different,and the content of glycerol trioleate in Semen Coicis which seeds from the same area is also different.
出处
《中国现代中药》
CAS
2011年第4期15-17,共3页
Modern Chinese Medicine
基金
国家科技攻关计划(2004BA721A21)
国家科技重大专项(2009ZX09308-002)
国家科技支撑计划(201107011)
