摘要
目的 建立同时测定鸦胆子药材中1,2-油酸-3-亚油酸甘油酯和甘油三油酸酯两种脂溶性成分含量的方法。方法 采用UPLC-ELSD法,色谱柱为Thermo Accucore XL C18柱（250 mm×2.1 mm,4μm）;流动相为乙腈-二氯甲烷（体积比70∶30）;流速为0.4 m L·min-1;柱温为30℃;进样量为4μL;漂移管温度为45℃;雾化器载气压为0.276 MPa。结果 1,2-油酸-3-亚油酸甘油酯和甘油三油酸酯进样量分别在0.509～2.55μg（r=0.999 6）和0.685～3.42μg（r=0.999 1）内线性关系良好,平均加样回收率分别为97.5%和99.5%,RSD分别为1.7%和2.3%（n=9）。结论 建立的方法可用于同时测定鸦胆子中1,2-油酸-3-亚油酸甘油酯和甘油三油酸酯的含量,为控制鸦胆子药材质量提供试验依据。
Abstract ： Objective To develop a method for the simultaneous determination of the contents of 1,2-olein-3- linolein and glycery trioleate in Brucea javanica by UPLC-ELSD. Methods The separation was carried out on a Thermo Accueore XL C18 colurrm（250 mm ×2. 1 mm,4 μm） with a mobile phase of acetonitrile and dichloromethane （ V： V = 70： 30）at a rate of 0. 4 mL·min- 1 ;the column temperature was 30 ℃ and the ELSD tube temperature was set at 45 ℃. Results The liner range of calibration curve was 0. 509 - 2. 55 μg （ r = 0.999 6 ） and 0.685 - 3.42 μg （ r = 0.999 1 ） for 1,2-olein-3-1inolein and glycery trioleate respectively. The average recovery was 97.5% and 99.5% with RSDs of 1.7% and 2.3% （ n = 9 ）, respectively. Conclusions The method can be applied for the simultaneous determination of 1,2-olein-3-1inolein and glyc- ery trioleate in Brucea javanica, and provide a reference for the quality control of Brucea javanica.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2016年第9期713-717,共5页
Journal of Shenyang Pharmaceutical University
关键词
鸦胆子
甘油三油酸酯
1
2-油酸-3-亚油酸甘油酯
超高效液相-蒸发光散射法
含量测定
Brucea javanica
glycery trioleate
1, 2-olein-3-1inolein
ultra performance liquid chromatography-evaporative light scattering detector（UPLC-ELSD）
content determination
