摘要
目的观察不同剂量四物合剂对马利兰诱导HL-60细胞凋亡作用的影响。方法 MTT法检测细胞增殖活性,流式细胞术检测细胞凋亡情况,Western blot检测细胞p53/bcl-2表达,ELISA法检测细胞色素C释放,分光光度法检测Caspase-3、Caspase-8和谷胱甘肽(GSH)。结果四物合剂可激活细胞Caspase-3和Caspase-8,上调细胞p53/bcl-2表达比率、升高细胞色素C释放、降低GSH(P<0.05,P<0.01),且60mg/L剂量可抑制HL-60细胞生长(P<0.05)。结论四物合剂可抑制HL-60细胞生长,对马利兰诱导HL-60细胞凋亡具有增效作用,其增效作用机制可能与激活肿瘤细胞凋亡PKC通路有关。
Objective To observant the dose effects of Siwu Mixture on the apoptosis in HL-60 leukemic cells by myleran.Methods HL-60 leukemic cells proliferation was tested by MTT assay,cell apoptosis was analyzed by flow cytometry,the p53/bcl-2 expression was assessed by Western blot,cytochrome C release was determined by ELISA,GSH,Caspase-3 and Caspase-8 activation were examined by colorrimetric assay.Results Siwu mixture could increase HL-60 leukemic cell apoptosis by myleran,upregulated ratio of p53/bcl-2 expression,activated of Caspase-3 and Caspase-8,increased the release of cytochrome C,decreased the levels of GSH（P0.05,P0.01）,and inhibited the growth of HL-60 leukemic cells at the dose of 60mg/L（P0.05）.Conclusion Siwu mixture was able to inhibit the growth of HL-60 leukemic cells,enhance antitumor effect of myleran and the major mechanism siwu mixture＇s promotion sensitivity of tumor cell to chemotherapeutic agents might be related to activat apoptosis signal pathway.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第3期261-264,共4页
Cancer Research on Prevention and Treatment
基金
漯河医学高等专科学校科研基金资助项目(2009-LMC-S-14)
漯河市科技进步奖励资助项目(2009518)
