摘要
目的建立高效毛细管电泳测定肌酐和尿酸的新方法。方法选择pH7．2、120mmol／LSDS、30mmol／L磷酸盐缓冲液体胶束电动毛细管电泳运行液，利用外标法定量，检测波光235um，同时测定尿肌酐和尿酸；非涂渍毛细管21cm×50μm（1．d）：电压10kV，自动进样2s，电泳6min。结果肌酐和尿酸的线性范围分别为7000～27.030μmol／L，3000～40.625μmol／L，最低检测限分别为9.190μmol／L和20.310μmol／L。建立了直接测定尿肌酐和尿酸两者浓度的毛细管电泳方法。本法的日内和日间变异系数都小于45％。肌酐和尿酸的平均回收率分别为102．7％和99.6％。内生化合物和常用药物对此方法无干扰。结论该方法线性范围宽，无需作样品稀释，简单、快速、可应用于临床样品检测。
Objective To develop a new method for simultaneous determination of creatinine and uric acid in human urine usingcapillary electrophoresis (CE). Methods Using pH7.2, 120 mmol/L SDS, 30 mmol/L phosphate buffer as electrophoresis buftbLuncoated fused-silica capillary 21 cm ×50 μm (i.d.) creatinine and uric acid were detected at the voltage of 10 kV at 235 urn, runningtime of 6 min, automatic injection by pressure. Results The linear concentration of creatinine and uric acid were 7 000-27.030μmol/L, 3 000～40.625 μmol/L, respectively. The average recoveries ofcreatinine and uric acid at different levels were found to be102.27% (n = 9) and 101.45% (n = 9), respectively. The lowest detection limit was 9. 19 μmol/L for creatinine and 20.7 μmol/L foruric acid. The precision stUdies of the assay both within one day and between days produced CV values less than 4.5%. Endogenouschemicals and commonly-used drugs showed no interference with this chromatographic procedure. Conclusion CE, which cansimultaneously determine creatinine and uric acid in human urine, is simple and rapid to perform.
出处
《第一军医大学学报》
CSCD
1999年第3期261-262,共2页
Journal of First Military Medical University
关键词
肌酐
尿酸
毛细管电泳法
尿液
creatinine, uric acid, urine, capillary electrophoresis
