摘要
目的：用高效毛细管电泳法测定硫酸长春地辛的含量。考察不同试验条件下硫酸长春地辛的胶束动电毛细管电泳行为。方法：选择５ｍｍｏｌ·Ｌ－１十二烷基硫酸钠、５０ｍｍｏｌ·Ｌ－１硼砂溶液（ｐＨ７７）为运行缓冲液，毛细管有效长度是５０ｃｍ（ＩＤ７５μｍ），以烟酰胺为内标，于２６０ｎｍ波长处测定。结果：在５８～５８０μｇ·ｍＬ－１浓度范围内呈良好线性，平均回收率达１００１％，ＲＳＤ为１８％（ｎ＝５）。
Objective:A new analysis method of vindensine sulfate was developed using micellaen electrekinetic capillary chromatography (MECC) by optimizing the major affecting factors.Methods:The running buffer solution was 50 m mol·L -1 borate buffer(pH 7 7) added 25 m mol·L -1 SDS.The internal standard was nicotiamide.The capillary column efficient length was 50 cm(ID 75).Electrophoresis was performed at 20 kV.The method showed good linearity( r =0 9999).Results:The concentration rang was 58～580 μg·mL -1 .The recovery and RSD were 100 1% and 1 8% ( n =5),respectively.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1999年第1期30-32,共3页
Chinese Journal of Pharmaceutical Analysis
