摘要
采用电穿孔基因转移技术向Ψ－２细胞内导入ＥＢＶＬｍｐ一ｎｅｏ基因，通过Ｇ４１８筛选获得８６个抗性克隆。用ＰＣＲ检测进一步确定了外源基因在抗Ｃ４１８克隆中稳定的整合。研究了部分电学参数对细胞存活及基因转移效率的影响以及电穿孔基因条件的优化。
his paper reported the introduction of pMV/LMP/C-myc-neo fusion gene into Ψ-2 cells byelectroporation. The 86 resistant colonies were obtained by selective medium which containedG4l8 sulfate;the stable integration of foreign gene(LMP)in Ψ-2 cells were determined by PCR.Several parameters of electroporation were investigated.The optimal electroporation conditionsand optimal efficiency of gene transfer were described.In summary,electroporation is a usefulmethod to obtain transient or stable expression of foreign genes in animal and plant cells.
出处
《湖南医科大学学报》
CSCD
1996年第5期459-462,共4页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
肿瘤
发生
基因治疗
电穿孔
EBV
Lmp基因
gene transfer
electroporation
EBV LMP gene
G418 resistant colonies
