摘要
目的:建立同时测定鱼腥草注射液中4-萜烯醇、α-松油醇、乙酸龙脑酯和甲基正壬酮含量的方法。方法:采用气相色谱法;色谱柱:DB-1毛细管气相色谱柱(30 m×0.25 mm,0.25μm);程序升温:70℃保持5 min,以5℃.min-1上升至140℃,保持5 min,再以20℃.min-1升至250℃;进样口温度:250℃;检测器:FID;检测器温度:280℃;内标:十一碳烯。结果:4-萜烯醇、α-松油醇、乙酸龙脑酯和甲基正壬酮浓度分别在24.75～742.5μg·mL-1(r=1.0000),12.9～387.0μg·mL-1(r=0.9999),20.4～612μg·mL-1(r=0.9999),25.35～760.5μg·mL-1(r=0.9999)范围内线性关系良好;平均回收率(n=6)分别为96.3%,95.2%,97.6%,98.8%,RSD分别为1.5%,1.1%,2.1%,2.1%。结论:该方法简便、快速、准确,可有效控制鱼腥草注射液质量。
Objective：To establish a method for simultaneous determination of 4-terpineol,α-terpineol,bornyl acetate,and methyl-n-nonylketone in Houttuynia cordata injection.Methods： GC determination was performed on a DB-1 column（30 m × 0.25 mm,0.25 μm）.The column temperature was maintained at 70 ℃ after injection for 5 min,and programmed to 140 ℃ at the rate of 5 ℃·min-1,then held for 5 min.After that the temperature was elevated to 250 ℃ at the rate of 20 ℃ · min-1.The FID was used as detector,the temperature of the injection and the detector were set at 250 ℃and 280 ℃,respectively.Undecylene was used as internal standard.Results：Good linearity of the four components were obtained within the range of 24.75-742.5 μg·mL-1（r=1.0000）,12.90-387.0 μg·mL-1（r=0.9999）,20.40-612.0 μg·mL-1（r=0.9999）,25.35-760.5 μg·mL-1（r=0.9999）,respectively.The average recoveries of 4-terpineol,α-terpineol,bornyl acetate,methyl-n-nonylketone were 96.3%,95.2%,97.6%,98.8% and the RSDs were 1.5%,1.1%,2.1%,2.1%,respectively.Conclusion：The method is simple,rapid,accurate,and can be used for the quality control of Houttuynia cordata injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第4期664-667,共4页
Chinese Journal of Pharmaceutical Analysis
