摘要
目的建立鱼腥草挥发油的气相色谱法,并用该法对不同产地鱼腥草挥发油中α-蒎烯、β-蒎烯、乙酸龙脑酯和甲基正壬酮进行同时的含量测定,根据含量水平对鱼腥草药材进行聚类分析。方法采用挥发油提取器,利用GC测定4种化学成分的含量。采用HP-5石英毛细管色谱柱(30 m×0.25 mm,0.25μm),程序升温,流速为1 mL.min-1,分流比为10∶1,进样量为1μL,采用SPSS 16.0统计软件进行聚类分析。结果α-蒎烯、β-蒎烯、乙酸龙脑酯和甲基正壬酮的线性范围分别为0.052～0.783 g.L-1、0.097～1.464 g.L-1、0.112～1.685 g.L-1、0.377～5.663 g.L-1,平均回收率分别为101.3%、97.3%、101.7%、96.9%,RSD分别为1.9%、1.2%、1.7%、1.6%,所分析的鱼腥草药材大致划分为两类。结论该方法准确、重复性好,可用于鱼腥草干品的质量控制。
Objective To establish a GC method of Houttuynia cordata volatile oil and develop this method for determination of α-pinene ,β-pinene, bornyl acetate and methyl nonylketone from different districts at the same time. Methods Oil extractor and GC were used to determine the four kinds of chemical components. HP-5 quartz capillary column （ 30m×0.25mm,0.25μm） programmed temperature was used at the flow rate of 1 mL.min-1 with split ratio of 10：1 ;injection volume was 1 μL. Cluster analysis was performed by SPSS 16.0 software. Results α-Pinene,β-pinene, bornyl acetate and methyl nonylketone were linear in the range of 0. 052-0. 783 g·L^-1 ,0. 097- 1. 464 g·L^-1,0. 112- 1. 685 g·L^-1,0. 377-5. 663 g·L^-1 ,the average recovery were 101.3% ,97.3% ,101.7% ,96.9% （RSD were 1.9% ,1.2% ,1.7% ,1.6% ） ,respectively. The samples analyzed were divided into two classes. Conclusions The method is accuracy and repeatability, and can be used for the quality control of dry goods Houttuynia cordata.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2011年第2期130-134,共5页
Journal of Shenyang Pharmaceutical University
