摘要
目的初步建立一种对肺炎克雷伯菌引起血流感染的快速检测方法。方法应用实时荧光定量PCR(Real-time PCR),从血培养阳性瓶中提取细菌基因组DNA,以特异性引物扩增16S rRNA靶片段,同期进行细菌培养鉴定的对照。结果检测142份已知肺炎克雷伯阳性标本,65份非肺炎克雷伯阴性对照,52份双盲样本显示敏感性为100%(146/146)特异性为100%(113/113),整个实验可在2h内完成。结论较传统的培养鉴定法而言,以16SrRNA作为实时荧光定量靶基因可快速检测肺炎克雷伯菌,具有很好的研究价值与应用前景。
Aim To establish a methods for rapid detection of bloodstream infections with Klebsiella pneumoniae. Methods Real-time fluorescence quantitative PCR（Real-time PCR）was used for detection of bacterial genomic DNA with amplified 16S rRNA target fragments in samples extracted from the blood culture positive bottles and compared to bacterial culture and identification of the control.Results The sensitivity and specificity of detection of 142 samples positive for Klebsiella pneumoniae,65 control samples negative for Klebsiella pneumoniae and 52 double-blind samples were all 100%（146/146）and 100%（113/113）.The entire experiment could be completed within 2 hours.Conclusions Compared to the traditional technique,Klebsiella pneumoniae can be rapidly detected with 16S rRNA as a real-time quantitative target genes,showing a promising prospect.
出处
《中国热带医学》
CAS
2011年第3期272-273,共2页
China Tropical Medicine
基金
陕西省卫生厅科学研究基金项目(H0813)
