摘要
利用定量逆转录聚合酶链反应（ＲＴ－ＰＣＲ）方法，研究了低浓度甲基硝基亚硝基胍（ＭＮＮＧ）对ＨｅＬａ细胞ＤＮＡ聚合酶（Ｐｏｌα，β，δ，ε）及拓扑异构酶Ⅱα（ＴｏｐⅡα）ｍＲＮＡ表达水平的影响．发现经０．２μｍｏｌ·Ｌ－１ＭＮＮＧ处理２．５ｈ后，ＨｅＬａ细胞ＰｏｌβｍＲＮＡ水平在６－２４ｈ内升高约１倍，而Ｐｏｌα，δ，ε及ＴｏｐＩαｍＲＮＡ水平则无明显改变．提示ＰｏｌβｍＲＮＡ水平改变可能参与ＭＮＮＧ诱发细胞遗传不稳定的发生．
It was reported from our laboratory that low concentration N methyl N′ nitro N nitrosoguanidine (MNNG, 0.2 μmol·L 1 ) could induce genetic instability in mammalian cells. To elucidate its mechanism, quantitative reverse transcription PCR (RT PCR) was used to study the effect of low concentration MNNG on mRNA expression of DNA polymerases and topoisomerase Ⅱα in HeLa cell. It was demonstrated that mRNA level of polymerase β increased by nearly one fold in HeLa cell from the 6th to 24th hour after being treated with 0.2 μmol·L 1 MNNG for 2.5 h, while there were no obvious mRNA expression alteration in polymerase α, δ, ε or topoisomerase Ⅱα. The above results suggest that the alteration of mRNA level of polymerase β be involved in the genesis of genetic instability induced by MNNG in mammalian cells.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1999年第3期217-221,共5页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然科学基金
省自然科学基金
关键词
甲基硝基
亚硝基胍
DNA
MNNG
聚合酶
基因表达
N methyl N' nitro N
nitrosoguanidine
DNA polymerases
gene expression
polymerase chain reaction
cell
HeLa
topoisomerase Ⅱα
mRNA
