摘要
为建立复方秋水仙碱胶囊中秋水仙碱的含量测定方法 ,采用高效液相色谱法 ,色谱柱Kromasil1 0 0～ 5C18( 5 μm ,4 6mm× 1 5 0mm) ,流动相为V( 0 0 5mol/L磷酸二氢钾 ) +V(甲醇 ) =5 0+5 0 ,流速 1 0mL/min ,紫外检测波长 3 5 0nm ,柱温 2 0℃ ,外标法定量。结果表明 ,秋水仙碱在3 0 5～ 9 1 5 μg/mL之间线性关系良好 ,回归方程为y =47 1 43 0x -1 3 82 7,r =0 9994(n =5 ) ,平均回收率为 1 0 0 1 9% ,RSD =0 86% (n=9)。本法简便、准确、可靠 ,可作为控制复方秋水仙碱胶囊质量标准的方法。
To establish a method for determination of the content of colchicine in compound colchicine capsules,a HPLC method was utilized.The sample was separated on a Kromasil 100～5 C 18 column (5 μm,4.6 mm×150 mm)and detected at 350 nm,using mixture of 0.05 mol/L potassium dihydrogen phosphate buffer-methanol (50∶50) as the mobile phase,the flow rate was 1.0 mL/min,the column temperature was kept at 20℃.The contents were calculated with external standard.The results showed that the calibration curve was linear within the range from 3.05 to 9.15 μg/mL,y=47.143 0 x-1.382 7,r=0.999 4(n=5),the average recovery was 100.19%,RSD=0.86%(n=9).It concludes this method is simple,accurate and reliable,and can be used to control the quality of compound colchicine capsules.
出处
《广东微量元素科学》
CAS
2004年第6期41-44,共4页
Trace Elements Science
