摘要
以复合蛋白酶为水解酶,研究了小麦面筋蛋白酶解前STP磷酸化处理对其酶解特性的影响.通过单因素试验和正交试验确定磷酸化处理的最佳工艺条件为:STP添加量为小麦面筋蛋白的9%,温度25℃,pH8.5,处理时间30 m in.同时研究了STP磷酸化处理后小麦面筋蛋白在酶解过程中的水解度和蛋白提取率的变化规律.
The paper studied the influence of the STP phosphate processing on the enzymatic hydrolysis characteristics of wheat gluten before enzymatic hydrolysis using a complex protease. Through singe factor experiments and orthogonal experiments, the paper determined the optimal conditions: the STP amount 9% , temperature 25 ℃ , pH 8.5, and reaction time 30 minutes. The paper also studied the change rule of the degree of hydrolysis and the protein extraction rate of the phosphate-processed wheat gluten during hydrolysis.
出处
《河南工业大学学报(自然科学版)》
CAS
北大核心
2011年第1期31-35,共5页
Journal of Henan University of Technology:Natural Science Edition
基金
国家自然科学基金项目(31071496)
河南省教育厅自然科学研究计划项目(2010A550001)
关键词
磷酸化处理
小麦面筋蛋白
酶解特性
水解度
phosphate processing
wheat gluten
enzymatic hydrolysis characteristics
degree of hydrolysis