摘要
目的 探讨肿瘤抑制基因ｎｍ２３－ Ｈ１ 与ＣＤ４４ 变异型（ＣＤ４４ｖ） 在口腔癌中的作用和意义。方法采用流式细胞术，对肿瘤转移抑制基因ｎｍ２３－ Ｈ１ 和ＣＤ４４ｖ 的表达量进行定量分析。结果ｎｍ２３ － Ｈ１ 抑制基因在口腔癌、白斑、扁平苔癣中表达的阳性率分别为５５ ％ ，１０ ％ 和７ ％ ；ＣＤ４４ｖ 在口腔癌、白斑、扁平苔癣中表达的阳性率分别为７５ ％ 、３０ ％ 和２１ ％ ；在ｎｍ２３－ Ｈ１ 基因的平均表达量中，口腔癌与白斑以及与扁平苔癣之间差异均有显著性（ Ｐ＜ ０ ．０１ 和Ｐ＜ ０．００５）；在口腔癌中，ｎｍ２３ － Ｈ１ 阴性表达的转移率为２８ ％ ，高于ｎｍ２３ － Ｈ１ 阳性表达的转移率５ ％ ，二者之间差异有显著性（ Ｐ＜ ０ ．０２）；在ＣＤ４４ｖ 的平均表达量中，口腔癌与口腔正常粘膜以及与白斑和扁平苔癣之间差异均有显著性（ Ｐ ＜ ０ ．００５ 和Ｐ ＜ ０．００１） ；在口腔癌中，ＣＤ４４ｖ 阳性表达的转移率为２０ ％ ，高于ＣＤ４４ｖ 阴性表达转移率０，两组间差异有显著性（ Ｐ ＜ ０．０１）。结论在口腔癌前病变发展为口腔癌的过程中，ｎｍ２３ － Ｈ１ 抑制基因和ＣＤ４４ｖ 均有显著表达；ｎｍ２３ － Ｈ１ 的低表达和ＣＤ４４ｖ 的高表?
ObjectiveTo explore the significance and role of tumor metastatic suppressor gene nm23-H1 and CD44 variant (VD44v)in tumorigenesis of the oral squamous cell carcinoma (SCC). Methodsnm23-H1 gene protein and CD44v protein expression in oral SCC and oral premalignant lesions were quantitively studied by flow cytometry. Resultsnm23-H1 expression was found in 55 % ,10 % and 7 % of oral SCC,leukoplakia and lichen planus, respectivly.CD44v expression was detected in 75 %,30 % and 21 % of the above diseases, respectively. The statistical significances of the fluorescence indexes(FI) in nm23-H1 expression were found between oral SCC and leukoplakia as well as lichen planus (P< 0.01 and P< 0.005 , respectively) in oral SCC.nm23-H1 lower expression tumors were associated with a higher rate of metastasis(28 %). The obvious different statistical significances of FI in CD44v expression were found between oral SCC and normal mucosa, leukoplakia as well as lichen planus (P< 0.05 , P< 0.005 and P< 0.001 ,respectively).CD44v higher expression tumors were associated with a higher rate of metastasis (20 %). ConclusionThere was obvious alteration of nm23-H1 and CD44v in the process of leukoplakia and lichen planus to oral SCC.nm23-H1 lower expression and CD44v higher expression may have an important significance in the metastasis of oral SCC.
出处
《河北医科大学学报》
CAS
1999年第6期321-324,共4页
Journal of Hebei Medical University
基金
"双十双百双千人才工程"科研基金
关键词
口腔肿瘤
肿瘤抑制基因
癌前病变
NM23-H1
mouth neoplasms/metab
precancerous conditions
oncogens proteins
flow cytometry/methods
