摘要
目的观察放射前后肺组织中两种炎症细胞因子的表达,明确放射性肺损伤的发生发展机制。方法 35只Balb/c健康雄性小鼠,随机分为照射组(全肺单次照射12 Gy)和对照组。各组于照射后15、30和90 d终止实验。肺组织行组织病理学检查及Masson染色、免疫组织化学染色观察TGF-β1、TNF-α表达情况。结果照射组照射野脱毛、皮肤可见湿疹,部分有糜烂。30 d时即出现广泛胶原纤维沉积。照射后肺组织TGF-β1、TNF-α表达强度均较对照组增高,且随时间延长表达增多(P<0.05)。结论 TGF-β1和TNF-α参与了放射性肺损伤的发生发展。
【Objectives】To observe the expression of two inflammatory cytokines before and after irradiation and approaching the pathogenesis of radiation-induced pulmonary injury.【Methods】35 male Balb/c mice were randomly divided into two groups：radiation alone（total lung single 12 Gy）,control group.Mice were sacrificed at the fifteenth,thirtieth and ninetieth day.Lung tissues were used for masson＇s stain and immunohistologic test of cytokine TGF-β1,TNF-α.【Results】Hair loss,skin rash,partly erosion in radiation alone group were shown.Extensive collagen deposition has been shown from the thirtieth day.The expression of TGF-β1 and TNF-α was increasing by time after irradiation in comparison with sham radiation（P 0.05）.【Conclusions】The predominant localization of TGF-β1 and TNF-α in areas of inflammatory cell infiltrates and fibrosis suggests involvement of these cytokines in the pathogenesis of radiation-induced pulmonary injury.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第24期3694-3697,共4页
China Journal of Modern Medicine
基金
湖南省科技厅资助一般项目(No:06SK3029-4)
